Specific bioanalytical optical and photoelectrochemical assays for detection of methanol in alcoholic beverages

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ISSN: 0956-5663
DOI: 10.1016/j.bios.2017.10.022
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2018-03-15
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Abstract
Methanol is a poison which is frequently discovered in alcoholic beverages. Innovative methods to detect methanol in alcoholic beverages are being constantly developed. We report for the first time a new strategy for the detection of methanol using fluorescence spectroscopy and photoelectrochemical (PEC) analysis. The analytical system is based on the oxidation of cysteine (CSH) with hydrogen peroxide (H2O2) enzymatically generated by alcohol oxidase (AOx). H2O2 oxidizes capping agent CSH, modulating the growth of CSH-stabilized cadmium sulphide quantum dots (CdS QDs). Disposable screen-printed carbon electrodes (SPCEs) modified with a conductive osmium polymer (Os-PVP) complex were employed to quantify resulting CdS QDs. This polymer facilitates the “wiring” of in situ enzymatically generated CdS QDs, which photocatalyze oxidation of 1-thioglycerol (TG), generating photocurrent as the readout signal. Likewise, we proved that our systems did not suffer from interference by ethanol. The PEC assays showed better sensitivity than conventional methods, covering a wide range of potential applications for methanol quantification.
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Publisher Copyright: © 2017 Elsevier B.V.
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Quantum dots , Methanol , Fluorescence , Photoelectrochemistry , Cysteine , Quantum dots , Methanol , Fluorescence , Photoelectrochemistry , Cysteine , Biotechnology , Biophysics , Biomedical Engineering , Electrochemistry , Funding Info , the Spanish Ministry of Economy and_x000D_ Competitiveness (project BIO2014-59741). , the Spanish Ministry of Economy and_x000D_ Competitiveness (project BIO2014-59741).
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journal article
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Barroso , J , Díez-Buitrago , B , Saa , L , Möller , M , Briz , N & Pavlov , V 2018 , ' Specific bioanalytical optical and photoelectrochemical assays for detection of methanol in alcoholic beverages ' , Biosensors and Bioelectronics , vol. 101 , pp. 116-122 . https://doi.org/10.1016/j.bios.2017.10.022