RT Journal Article
T1 Specific bioanalytical optical and photoelectrochemical assays for detection of methanol in alcoholic beverages
A1 Barroso, Javier
A1 Díez-Buitrago, Beatriz
A1 Saa, Laura
A1 Möller, Marco
A1 Briz, Nerea
A1 Pavlov, Valeri
AB Methanol is a poison which is frequently discovered in alcoholic beverages. Innovative methods to detect methanol in alcoholic beverages are being constantly developed. We report for the first time a new strategy for the detection of methanol using fluorescence spectroscopy and photoelectrochemical (PEC) analysis. The analytical system is based on the oxidation of cysteine (CSH) with hydrogen peroxide (H2O2) enzymatically generated by alcohol oxidase (AOx). H2O2 oxidizes capping agent CSH, modulating the growth of CSH-stabilized cadmium sulphide quantum dots (CdS QDs). Disposable screen-printed carbon electrodes (SPCEs) modified with a conductive osmium polymer (Os-PVP) complex were employed to quantify resulting CdS QDs. This polymer facilitates the “wiring” of in situ enzymatically generated CdS QDs, which photocatalyze oxidation of 1-thioglycerol (TG), generating photocurrent as the readout signal. Likewise, we proved that our systems did not suffer from interference by ethanol. The PEC assays showed better sensitivity than conventional methods, covering a wide range of potential applications for methanol quantification.
SN 0956-5663
YR 2018
FD 2018-03-15
LA eng
NO Barroso , J , Díez-Buitrago , B , Saa , L , Möller , M , Briz , N & Pavlov , V 2018 , ' Specific bioanalytical optical and photoelectrochemical assays for detection of methanol in alcoholic beverages ' , Biosensors and Bioelectronics , vol. 101 , pp. 116-122 . https://doi.org/10.1016/j.bios.2017.10.022
NO Publisher Copyright: © 2017 Elsevier B.V.
DS TECNALIA Publications
RD 3 jul 2024