Specific bioanalytical optical and photoelectrochemical assays for detection of methanol in alcoholic beverages

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dc.contributor.authorBarroso, Javier
dc.contributor.authorDíez-Buitrago, Beatriz
dc.contributor.authorSaa, Laura
dc.contributor.authorMöller, Marco
dc.contributor.authorBriz, Nerea
dc.contributor.authorPavlov, Valeri
dc.contributor.institutionTecnalia Research & Innovation
dc.contributor.institutionSG
dc.date.issued2018-03-15
dc.descriptionPublisher Copyright: © 2017 Elsevier B.V.
dc.description.abstractMethanol is a poison which is frequently discovered in alcoholic beverages. Innovative methods to detect methanol in alcoholic beverages are being constantly developed. We report for the first time a new strategy for the detection of methanol using fluorescence spectroscopy and photoelectrochemical (PEC) analysis. The analytical system is based on the oxidation of cysteine (CSH) with hydrogen peroxide (H2O2) enzymatically generated by alcohol oxidase (AOx). H2O2 oxidizes capping agent CSH, modulating the growth of CSH-stabilized cadmium sulphide quantum dots (CdS QDs). Disposable screen-printed carbon electrodes (SPCEs) modified with a conductive osmium polymer (Os-PVP) complex were employed to quantify resulting CdS QDs. This polymer facilitates the “wiring” of in situ enzymatically generated CdS QDs, which photocatalyze oxidation of 1-thioglycerol (TG), generating photocurrent as the readout signal. Likewise, we proved that our systems did not suffer from interference by ethanol. The PEC assays showed better sensitivity than conventional methods, covering a wide range of potential applications for methanol quantification.en
dc.description.statusPeer reviewed
dc.format.extent7
dc.format.extent802456
dc.identifier.citationBarroso , J , Díez-Buitrago , B , Saa , L , Möller , M , Briz , N & Pavlov , V 2018 , ' Specific bioanalytical optical and photoelectrochemical assays for detection of methanol in alcoholic beverages ' , Biosensors and Bioelectronics , vol. 101 , pp. 116-122 . https://doi.org/10.1016/j.bios.2017.10.022
dc.identifier.doi10.1016/j.bios.2017.10.022
dc.identifier.issn0956-5663
dc.identifier.otherresearchoutputwizard: 11556/503
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85031490285&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofBiosensors and Bioelectronics
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subject.keywordsQuantum dots
dc.subject.keywordsMethanol
dc.subject.keywordsFluorescence
dc.subject.keywordsPhotoelectrochemistry
dc.subject.keywordsCysteine
dc.subject.keywordsQuantum dots
dc.subject.keywordsMethanol
dc.subject.keywordsFluorescence
dc.subject.keywordsPhotoelectrochemistry
dc.subject.keywordsCysteine
dc.subject.keywordsBiotechnology
dc.subject.keywordsBiophysics
dc.subject.keywordsBiomedical Engineering
dc.subject.keywordsElectrochemistry
dc.subject.keywordsFunding Info
dc.subject.keywordsthe Spanish Ministry of Economy and_x000D_ Competitiveness (project BIO2014-59741).
dc.subject.keywordsthe Spanish Ministry of Economy and_x000D_ Competitiveness (project BIO2014-59741).
dc.titleSpecific bioanalytical optical and photoelectrochemical assays for detection of methanol in alcoholic beveragesen
dc.typejournal article
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