Fast Electrochemical Measurement of Laccase Activity for Monitoring Grapes’ Infection with Botrytis cinerea
Loading...
Identifiers
Publication date
2022-03-15
Advisors
Journal Title
Journal ISSN
Volume Title
Publisher
Citations
Export
Abstract
Grapes’ infection with the fungi Botrytis cinerea is one of the major causes of economic loss in the winemaking sector worldwide. The laccase activity of grapes is considered an appropriate indicator of this type of fungal infection, and enzymatic activity higher than 3 U/mL indicates a high risk of irreversibly damaged grape must due to enzymatic browning. This work describes a fast test for the measurement of laccase activity based on a dual optical and electrochemical detection method. A paper sensor impregnated with the enzymatic substrate dye 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) provides a semi-quantitative optical measurement. While the paper sensor can be used independently, when combined with a screen-printed electrode and amperometry measurements, it enables the quantitative detection of laccase activities down to 0.4 U/mL in only 5 min. The method was applied for monitoring the artificial infection of white, rosé, and red grapes with different strains of Botrytis cinerea. The results were confirmed by parallel analysis using the spectrophotometric method of laccase activity determination based on syringaldazine. The influence of the fungal strain and type of grape on laccase activity levels is reported. The demonstrated robustness, simplicity, and versatility of the developed method make it ideal for application on-site in the vineyard or at grape processing points.
Description
Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
Keywords
Botrytis cinerea , Laccase , ABTS , Amperometry , Grapes , Botrytis cinerea , Laccase , ABTS , Amperometry , Grapes , Bioengineering , Chemical Engineering (miscellaneous) , Process Chemistry and Technology , Funding Info , Financial support provided by the Romanian Executive Agency for Higher Education, Research, Development and Innovation (UEFISCDI), ERANET-MANUNET-III-WINBIOTOOL-2, contract 151/9.03.2020 grant (for A.V), _x000D_ contract150/9.03.2020 (for P.E) and contract 152/9.03.2020 (for C.P. (Catalina Pantazi), E.B., and M.I.); _x000D_ the Romanian Academy grant RO1567-IBB05/2021 (for R.R. and C.P. (Cristina Purcarea)); _x000D_ the Basque Government and the European Union through the European regional development fund 2014–2020 (FEDER) (ZL-2020/00532 and ZL-2021/00340); _x000D_ and the Diputación Foral de Álava (ALAVA INNOVA program—INNOEM-2020/00045) for Bodegas de los Herederos del Marqués de Riscal; and the Diputación Foral de Álava (ALAVA INNOVA program— INNOEM-2020/00045) for Bodegas de los Herederos del Marqués de Risca is gratefully acknowledged. , Financial support provided by the Romanian Executive Agency for Higher Education, Research, Development and Innovation (UEFISCDI), ERANET-MANUNET-III-WINBIOTOOL-2, contract 151/9.03.2020 grant (for A.V), _x000D_ contract150/9.03.2020 (for P.E) and contract 152/9.03.2020 (for C.P. (Catalina Pantazi), E.B., and M.I.); _x000D_ the Romanian Academy grant RO1567-IBB05/2021 (for R.R. and C.P. (Cristina Purcarea)); _x000D_ the Basque Government and the European Union through the European regional development fund 2014–2020 (FEDER) (ZL-2020/00532 and ZL-2021/00340); _x000D_ and the Diputación Foral de Álava (ALAVA INNOVA program—INNOEM-2020/00045) for Bodegas de los Herederos del Marqués de Riscal; and the Diputación Foral de Álava (ALAVA INNOVA program— INNOEM-2020/00045) for Bodegas de los Herederos del Marqués de Risca is gratefully acknowledged.
Type
Citation
Lulea , A C , Ruginescu , R , Banciu , R M , Pantazi , C , Brinduse , E , Ion , M , Quintela , S , Elejalde , E , Fernández-de-Castro , L , Villarán , M C , Ruiz-de-Vergara , Z , Ruíz , C , Epure , P , Purcarea , C & Vasilescu , A 2022 , ' Fast Electrochemical Measurement of Laccase Activity for Monitoring Grapes’ Infection with Botrytis cinerea ' , Processes , vol. 10 , no. 3 , 575 , pp. 575 . https://doi.org/10.3390/pr10030575