RT Journal Article
T1 Fast Electrochemical Measurement of Laccase Activity for Monitoring Grapes’ Infection with Botrytis cinerea
A1 Lulea, Andreea Catalina
A1 Ruginescu, Robert
A1 Banciu, Roberta Maria
A1 Pantazi, Catalina
A1 Brinduse, Elena
A1 Ion, Marian
A1 Quintela, Silvia
A1 Elejalde, Edurne
A1 Fernández-de-Castro, Laura
A1 Villarán, Maria Carmen
A1 Ruiz-de-Vergara, Zuria
A1 Ruíz, Cristobal
A1 Epure, Petru
A1 Purcarea, Cristina
A1 Vasilescu, Alina
AB Grapes’ infection with the fungi Botrytis cinerea is one of the major causes of economic loss in the winemaking sector worldwide. The laccase activity of grapes is considered an appropriate indicator of this type of fungal infection, and enzymatic activity higher than 3 U/mL indicates a high risk of irreversibly damaged grape must due to enzymatic browning. This work describes a fast test for the measurement of laccase activity based on a dual optical and electrochemical detection method. A paper sensor impregnated with the enzymatic substrate dye 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) provides a semi-quantitative optical measurement. While the paper sensor can be used independently, when combined with a screen-printed electrode and amperometry measurements, it enables the quantitative detection of laccase activities down to 0.4 U/mL in only 5 min. The method was applied for monitoring the artificial infection of white, rosé, and red grapes with different strains of Botrytis cinerea. The results were confirmed by parallel analysis using the spectrophotometric method of laccase activity determination based on syringaldazine. The influence of the fungal strain and type of grape on laccase activity levels is reported. The demonstrated robustness, simplicity, and versatility of the developed method make it ideal for application on-site in the vineyard or at grape processing points.
SN 2227-9717
YR 2022
FD 2022-03-15
LA eng
NO Lulea , A C , Ruginescu , R , Banciu , R M , Pantazi , C , Brinduse , E , Ion , M , Quintela , S , Elejalde , E , Fernández-de-Castro , L , Villarán , M C , Ruiz-de-Vergara , Z , Ruíz , C , Epure , P , Purcarea , C & Vasilescu , A 2022 , ' Fast Electrochemical Measurement of Laccase Activity for Monitoring Grapes’ Infection with Botrytis cinerea ' , Processes , vol. 10 , no. 3 , 575 , pp. 575 . https://doi.org/10.3390/pr10030575
NO Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
DS TECNALIA Publications
RD 4 jul 2024