Effect of high content nanohydroxyapatite composite scaffolds prepared via melt extrusion additive manufacturing on the osteogenic differentiation of human mesenchymal stromal cells

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dc.contributor.authorCámara-Torres, Maria
dc.contributor.authorSinha, Ravi
dc.contributor.authorSanchez, Alberto
dc.contributor.authorHabibovic, Pamela
dc.contributor.authorPatelli, Alessandro
dc.contributor.authorMota, Carlos
dc.contributor.authorMoroni, Lorenzo
dc.contributor.institutionECOEFICIENCIA DE PRODUCTOS DE CONSTRUCCIÓN
dc.date.issued2022-06
dc.descriptionPublisher Copyright: © 2022 The Authors
dc.description.abstractThe field of bone tissue engineering seeks to mimic the bone extracellular matrix composition, balancing the organic and inorganic components. In this regard, additive manufacturing (AM) of high content calcium phosphate (CaP)-polymer composites holds great promise towards the design of bioactive scaffolds. Yet, the biological performance of such scaffolds is still poorly characterized. In this study, melt extrusion AM (ME-AM) was used to fabricate poly(ethylene oxide terephthalate)/poly(butylene terephthalate) (PEOT/PBT)-nanohydroxyapatite (nHA) scaffolds with up to 45 wt% nHA, which presented significantly enhanced compressive mechanical properties, to evaluate their in vitro osteogenic potential as a function of nHA content. While osteogenic gene upregulation and matrix mineralization were observed on all scaffold types when cultured in osteogenic media, human mesenchymal stromal cells did not present an explicitly clear osteogenic phenotype, within the evaluated timeframe, in basic media cultures (i.e. without osteogenic factors). Yet, due to the adsorption of calcium and inorganic phosphate ions from cell culture media and simulated body fluid, the formation of a CaP layer was observed on PEOT/PBT-nHA 45 wt% scaffolds, which is hypothesized to account for their bone forming ability in the long term in vitro, and osteoconductivity in vivo.en
dc.description.statusPeer reviewed
dc.format.extent1
dc.format.extent16421828
dc.identifier.citationCámara-Torres , M , Sinha , R , Sanchez , A , Habibovic , P , Patelli , A , Mota , C & Moroni , L 2022 , ' Effect of high content nanohydroxyapatite composite scaffolds prepared via melt extrusion additive manufacturing on the osteogenic differentiation of human mesenchymal stromal cells ' , Biomaterials Advances , vol. 137 , 212833 , pp. 212833 . https://doi.org/10.1016/j.bioadv.2022.212833
dc.identifier.doi10.1016/j.bioadv.2022.212833
dc.identifier.issn2772-9508
dc.identifier.otherresearchoutputwizard: 11556/1367
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85132554892&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofBiomaterials Advances
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subject.keywordsMelt extrusion additive manufacturing
dc.subject.keywordsNanohydroxyapatite
dc.subject.keywordsComposite
dc.subject.keywordsHuman mesenchymal stromal cells
dc.subject.keywordsOsteogenic differentiation
dc.subject.keywordsBone regeneration
dc.subject.keywordsMelt extrusion additive manufacturing
dc.subject.keywordsNanohydroxyapatite
dc.subject.keywordsComposite
dc.subject.keywordsHuman mesenchymal stromal cells
dc.subject.keywordsOsteogenic differentiation
dc.subject.keywordsBone regeneration
dc.subject.keywordsBiomedical Engineering
dc.subject.keywordsBiomaterials
dc.subject.keywordsBioengineering
dc.subject.keywordsProject ID
dc.subject.keywordsinfo:eu-repo/grantAgreement/EC/H2020/685825/EU/Functionally graded Additive Manufacturing scaffolds by hybrid manufacturing/FAST
dc.subject.keywordsinfo:eu-repo/grantAgreement/EC/H2020/685825/EU/Functionally graded Additive Manufacturing scaffolds by hybrid manufacturing/FAST
dc.subject.keywordsFunding Info
dc.subject.keywordsWe are grateful to H2020-NMP-PILOTS-2015 (GA n. 685825) for financial support. PH gratefully acknowledges the Gravitation Program ‘Materials-Driven Regeneration’, funded by the Netherlands Organization for Scientific Research (NWO) (024.003.013). Some of the materials used in this work were provided by the Texas A&M Health Science Center College of Medicine Institute for Regenerative Medicine at Scott & White through a grant from NCRR of the NIH (Grant #P40RR017447). We would also like to thank Eva Gubbins from MERLN Institute for performing the ICP-MS measurements.
dc.subject.keywordsWe are grateful to H2020-NMP-PILOTS-2015 (GA n. 685825) for financial support. PH gratefully acknowledges the Gravitation Program ‘Materials-Driven Regeneration’, funded by the Netherlands Organization for Scientific Research (NWO) (024.003.013). Some of the materials used in this work were provided by the Texas A&M Health Science Center College of Medicine Institute for Regenerative Medicine at Scott & White through a grant from NCRR of the NIH (Grant #P40RR017447). We would also like to thank Eva Gubbins from MERLN Institute for performing the ICP-MS measurements.
dc.titleEffect of high content nanohydroxyapatite composite scaffolds prepared via melt extrusion additive manufacturing on the osteogenic differentiation of human mesenchymal stromal cellsen
dc.typejournal article
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