Browsing by Author "Mendibil, Unai"
Now showing 1 - 2 of 2
Results Per Page
Sort Options
Item Decellularization of xenografted tumors provides cell-specific in vitro 3D environment(2022-08-18) Iazzolino, Gaia; Mendibil, Unai; Arnaiz, Blanca; Ruiz-de-Angulo, Ane; Azkargorta, Mikel; Uribe, Kepa B.; Khatami, Neda; Elortza, Felix; Olalde, Beatriz; Gomez-Vallejo, Vanessa; Llop, Jordi; Abarrategi, Ander; BiomaterialesIn vitro cell culture studies are common in the cancer research field, and reliable biomimetic 3D models are needed to ensure physiological relevance. In this manuscript, we hypothesized that decellularized xenograft tumors can serve as an optimal 3D substrate to generate a top-down approach for in vitro tumor modeling. Multiple tumor cell lines were xenografted and the formed solid tumors were recovered for their decellularization by several techniques and further characterization by histology and proteomics techniques. Selected decellularized tumor xenograft samples were seeded with the HCC1806 human triple-negative breast cancer (TNBC) basal-like subtype cell line, and cell behavior was compared among them and with other control 2D and 3D cell culture methods. A soft treatment using Freeze-EDTA-DNAse allows proper decellularization of xenografted tumor samples. Interestingly, proteomic data show that samples decellularized from TNBC basal-like subtype xenograft models had different extracellular matrix (ECM) compositions compared to the rest of the xenograft tumors tested. The in vitro recellularization of decellularized ECM (dECM) yields tumor-type–specific cell behavior in the TNBC context. Data show that dECM derived from xenograft tumors is a feasible substrate for reseeding purposes, thereby promoting tumor-type–specific cell behavior. These data serve as a proof-of-concept for further potential generation of patient-specific in vitro research models.Item Tissue-Specific Decellularization Methods: Rationale and Strategies to Achieve Regenerative Compounds(Multidisciplinary Digital Publishing Institute (MDPI), 2020-07-30) Mendibil, Unai; Ruiz-Hernandez, Raquel; Retegi-Carrion, Sugoi; Garcia-Urquia, Nerea; Olalde-Graells, Beatriz; Abarrategi, AnderThe extracellular matrix (ECM) is a complex network with multiple functions, including specific functions during tissue regeneration. Precisely, the properties of the ECM have been thoroughly used in tissue engineering and regenerative medicine research, aiming to restore the function of damaged or dysfunctional tissues. Tissue decellularization is gaining momentum as a technique to obtain potentially implantable decellularized extracellular matrix (dECM) with well-preserved key components. Interestingly, the tissue-specific dECM is becoming a feasible option to carry out regenerative medicine research, with multiple advantages compared to other approaches. This review provides an overview of the most common methods used to obtain the dECM and summarizes the strategies adopted to decellularize specific tissues, aiming to provide a helpful guide for future research development.