Browsing by Author "Goikoetxea, L."
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Item Bone cell adhesion on ion implanted titanium alloys(2005-06-22) Braceras, I.; Onate, J.I.; Goikoetxea, L.; Viviente, J.L.; Alava, J.I.; de Maeztu, M.A.; Tecnalia Research & Innovation; Biomateriales; TECNOLOGÍA DE MEMBRANAS E INTENSIFICACIÓN DE PROCESOSThe authors have previously reported than ion implantation can have a significant effect on osseointegration of an implant, specially when the latter is introduced in areas of poorer bone density. These results indicate that this process is particularly suited for implant devices introduced in elderly patients or in those regions that have a poor quality of bone. The aim of this work is directed to study osteoblast adhesion on Ti alloy surfaces with different ion implantation treatments, so osseoconductive properties of several surfaces can be assessed. Polished discs of Ti–6Al–4V and Ti CP GR1 titanium alloy have been prepared and ion implanted with different species and parameters (dose and energy). Afterwards, the samples have been sterilized by UV light, inoculated with 1.5×105 human bone cells and incubated during 4 h at 37 C and 5% CO2 atmosphere. Then, once fixed and rinsed, image analysis has been used to quantify the number of cells attached to the Ti discs. On a second round of tests, cell proliferation tests have been conducted during 24, 48, 144 and 192 h, respectively. Furthermore, surface analysis techniques (e.g. AFM) have been applied to learn about the qualitative behavior, i.e. morphology, of the attached cells. Cell attachment has shown to be highly sensitive to ion implantation parameters. Although some quantitative differences have been observed, the more significant differences were qualitative. AFM analysis has shown that the star-shaped bone cells attached spread more and occupied larger surfaces like in osseointegration prone surfaces, most probably due to extracellular matrix synthesized around them, while other surfaces showed mainly large and narrow shaped or round shaped bone cells often with great cellular nucleus in the middle of the cells and little extracellular matrix around. So, ion implanted surfaces that facilitate osseointegration have been identified, in terms of initial bone cell attachment quality, where although the number of attached cells were not necessarily always larger, they tended to occupy wider areas with healthier cells.Item In vitro evaluation of combined laser processing on Ti6Al4V discs: Macrostructuring and pulsed laser deposition(2008) Paz, M. D.; Chiussi, S.; González, P.; Serra, J.; León, B.; Goikoetxea, L.; Alava, J. I.; BiomaterialesHuman fetal osteoblast (hFOB) cell adhesion and cell proliferation studies have been performed on laser macrostructured titanium alloy substrates with and without pulsed laser deposited thin calcium phosphate coating. Coating was only 100 nm thick. The combined laser processing approach for modification of titanium alloy surfaces has yielded improved cell adhesion and proliferation of cells. Alkaline phosphatase activity was very significantly higher in the coated samples suggesting a intense stimuli of early osteoblast differentiation that should lead to improve bone growth on these surfaces.Item Interaction of engineered surfaces with the living world: Ion implantation vs. osseointegration(2007-08-05) Braceras, I.; Alava, J. I.; Goikoetxea, L.; de Maeztu, M. A.; Onate, J. I.; Biomateriales; Tecnalia Research & InnovationThe reaction of living tissues to foreign materials is a highly complex process that currently is insufficiently understood. Nevertheless, if specific reactions are to be promoted, this understanding is highly valuable and thus a significant research effort is being devoted to this issue. Typically, when a biomaterial is inserted in living tissue, proteins and other bio-molecules will adsorb to the surface. As this protein layer will mediate the interaction of the biomaterial with the living world, the consequent reactions will be highly dependant on this very first stage. Furthermore, different materials, i.e. surfaces, typically elicit a very different tissue response. It is commonly admitted that the primary adsorption depends heavily on the surface chemistry, surface topography and surface physical characteristics. Interactions between surface micro-topography and living cells have been widely studied, but protein specific reactions versus nano-topography have been barely explored. Ion beam modification of surfaces, which affect these key properties, can therefore be (i) a powerful tool to advance in the understanding of these nanoscale phenomena and (ii) useful as an industrial treatment of high value-added medical devices. This work will explore the application of ion beam based surface treatments to cause specific reactions in hard tissue regeneration. A variety of in vitro and in vivo results are presented corresponding to ion implantation treatments promoting "osseointegration" or intimate binding between the biomaterial and the living tissue, without any soft tissue interlayer, and an overview of the mechanism behind is offered, i.e. among other behaviour of osteoblasts, signalling proteins as the integrins, nanotopographic parameters.Item Nanozirconia partially coated MWNT: Nanostructural characerization and cytotoxicity and lixivation study(2008) Garmendia, N.; Bilbao, L.; Muñoz, R.; Goikoetxea, L.; García, A.; Bustero, I.; Olalde, B.; Garagorri, N.; Obieta, I.; PRINTEX; Biomateriales; Tecnalia Research & InnovationCarbon nanotubes could avoid the crack propagation and enhance the toughness of the ceramic material used for prostheses applications. So nanozirconia partially coated carbon nanotubes have been obtained via hydrothermal synthesis of zirconia nanoparticles in presence of multiwall carbon nanotubes. The as covered nanotubes should have a better wettability in the ceramic matrix and improve the dispersion of the CNTs in the nanocomposite, which results in a new ceramic biomaterial with a longer lifetime and better reliability. The obtained product has been structurally characterized by several techniques such as FTIR, XRD, SEM, AFM, EELS, XPS and TGA. The citotoxicity of the sintered product was studied by the change in the pH and ICP-AES in in-vitro biocompatibility tests.