Browsing by Author "Garcia-Urquia, Nerea"
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Item Acellular human corneal matrix sheets seeded with human adipose-derived mesenchymal stem cells integrate functionally in an experimental animal model(2015-03-01) Alio del Barrio, Jorge L.; Chiesa, Massimo; Garagorri, Nerea; Garcia-Urquia, Nerea; Fernandez-Delgado, Jorge; Bataille, Laurent; Rodriguez, Alejandra; Arnalich-Montiel, Francisco; Zarnowski, Tomasz; Álvarez de Toledo, Juan P.; Alio, Jorge L.; De Miguel, Maria P.; Tecnalia Research & Innovation; BiomaterialesPurpose: To evaluate the invivo biocompatibility of grafts composed of sheets of decellularized human corneal stroma with or without the recellularization of human adipose derived adult stem cells (h-ADASC) into the rabbit cornea. Methods: Sheets of human corneal stroma of 90μm thickness were decellularized, and their lack of cytotoxicity was assayed. The recellularization was achieved by the injection of 2×105 labeled h-ADASC in the graft followed by five days of cell culture. The grafts were implanted invivo into a stromal pocket at 50% depth. After a triple-masked three-month follow-up, the animals were euthanized and the biointegration of the graft, the viability of the stem cells and the expression of keratocan (human keratocyte-specific protein) were assessed. Results: The decellularized stromal sheets showed an intact extracellular matrix with a decellularization rate of 92.8% and an excellent recellularization capacity invitro with h-ADASC. A complete and stable graft transparency was observed during the full follow-up, with absence of any clinical sign of rejection. The postmortem analysis demonstrated the survival of the transplanted human stem cells inside the graft and their differentiation into functional keratocytes, as assessed by the expression of human keratocan. Conclusions: We report a new model of lamellar keratoplasty that requires only a simple and safe procedure of liposuction and a donor allogeneic cornea to provide an optically transparent autologous stromal graft with excellent biocompatibility and integration into the host tissue in a rabbit model.Item Tissue-Specific Decellularization Methods: Rationale and Strategies to Achieve Regenerative Compounds(Multidisciplinary Digital Publishing Institute (MDPI), 2020-07-30) Mendibil, Unai; Ruiz-Hernandez, Raquel; Retegi-Carrion, Sugoi; Garcia-Urquia, Nerea; Olalde-Graells, Beatriz; Abarrategi, AnderThe extracellular matrix (ECM) is a complex network with multiple functions, including specific functions during tissue regeneration. Precisely, the properties of the ECM have been thoroughly used in tissue engineering and regenerative medicine research, aiming to restore the function of damaged or dysfunctional tissues. Tissue decellularization is gaining momentum as a technique to obtain potentially implantable decellularized extracellular matrix (dECM) with well-preserved key components. Interestingly, the tissue-specific dECM is becoming a feasible option to carry out regenerative medicine research, with multiple advantages compared to other approaches. This review provides an overview of the most common methods used to obtain the dECM and summarizes the strategies adopted to decellularize specific tissues, aiming to provide a helpful guide for future research development.