Oliver, LauraFernández-de-Castro, LauraDietrich, ThomasVillaran, Maria CarmenBarrio, Ramón J.2022-04-14Oliver , L , Fernández-de-Castro , L , Dietrich , T , Villaran , M C & Barrio , R J 2022 , ' Production of Docosahexaenoic Acid and Odd-Chain Fatty Acids by Microalgae Schizochytrium limacinum Grown on Waste-Derived Volatile Fatty Acids ' , Applied Sciences , vol. 12 , no. 8 , 3976 , pp. 3976 . https://doi.org/10.3390/app120839762076-3417researchoutputwizard: 11556/1360Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.Heterotrophic microalgae are recognized as a source of bioactive compounds. However, there are still some drawbacks for their use at an industrial scale associated with the high cost of glucose, the main carbon source in heterotrophic cultures. In recent years, significant efforts have been made to investigate more sustainable carbon sources to produce biomass. In this study, the capacity of Schizochytrium limacinum to grow on waste-derived volatile fatty acids and the effect that their use produces on biomass and fatty acids profiles were investigated. Acetic, propionic, butyric, valeric and caproic acid were evaluated independently, as well as in a synthetic mixture (VFA). The use of acetic and butyric resulted in a good biomass productivity, while the use of valeric and propionic acid resulted in higher content of odd-chain fatty acids (OCFA), increasingly investigated due to their potential benefits for human health. The use of industrial waste-derived VFA as a potential carbon source was validated through the utilization of biowaste derived effluents from a volatile fatty acid platform. The biomass produced was of 18.5 g/L, 54.0% lipids, 46.3% docosahexaenoic acid (DHA) and 25.0% OCFA, concluding that waste derived VFA can produce DHA and OCFA in a suitable ratio of DHA/OCFA with potential industrial applications.11859251enginfo:eu-repo/semantics/openAccessjournal article10.3390/app12083976Heterotrophic microalgaeVolatile fatty acidsDHAOdd-chain fatty acidsHeterotrophic microalgaeVolatile fatty acidsDHAOdd-chain fatty acidsGeneral Materials ScienceInstrumentationGeneral EngineeringProcess Chemistry and TechnologyComputer Science ApplicationsFluid Flow and Transfer ProcessesSDG 9 - Industry, Innovation, and InfrastructureSDG 12 - Responsible Consumption and ProductionProject IDinfo:eu-repo/grantAgreement/EC/H2020/720777/EU/Biowaste derived volatile fatty acid platform for biopolymers, bioactive compounds and chemical building blocks/VOLATILEinfo:eu-repo/grantAgreement/EC/H2020/720777/EU/Biowaste derived volatile fatty acid platform for biopolymers, bioactive compounds and chemical building blocks/VOLATILEFunding InfoThe authors would like to thank the European project VOLATILE. The project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 720777.The authors would like to thank the European project VOLATILE. The project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 720777.http://www.scopus.com/inward/record.url?scp=85129022838&partnerID=8YFLogxK