Martínez-Pérez, PaulaPonce-Alcántara, SalvadorMurillo, NievesPérez-Márquez, AnaMaudes, JonPeraile, InésGonzález-López, LauraGil-García, MatildeLorenzo-Lozano, PalomaGarcía-Rupérez, JaimeFixler, DrorGoldys, Ewa M.Wachsmann-Hogiu, Sebastian2021Martínez-Pérez , P , Ponce-Alcántara , S , Murillo , N , Pérez-Márquez , A , Maudes , J , Peraile , I , González-López , L , Gil-García , M , Lorenzo-Lozano , P & García-Rupérez , J 2021 , Low-cost electrospun nanofibers for label-free and real-time optical biodetection purposes . in D Fixler , E M Goldys & S Wachsmann-Hogiu (eds) , Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVIII . , 116580E , Proceedings of SPIE - The International Society for Optical Engineering , vol. 11658 , SPIE , Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVIII 2021 , Vierual, Online , United States , 6/03/21 . https://doi.org/10.1117/12.2576527conference97815106415180277-786XPublisher Copyright: © 2021 SPIE. All rights reserved.In the development of label-free and real-time optical biosensors, a straightforward and low-cost fabrication of the transducer is important, because it reduces costs and the complexity of the process. One possibility for such easy fabrication is electrospinning. It is a versatile and well-developed technique that allows the manufacturing of layers of nanofibers at a low cost with a Fabry-Pérot cavity-based optical response when illuminated with visible light. In this research, the suitability of such layers for the development of label-free and real-time optical biosensors is studied. For such aim, the nanofibers were biofunctionalized in flow with antibodies against bovine serum albumin through an intermediate layer of protein A/G. Then, BSA was flowed at a concentration of 10 μg/ml as the target analyte. As a result, biofunctionalization and biodetection processes were optically monitored in real-time successfully, demonstrating the suitability of such a simple-to-fabricate transducer for the development of label-free and real-time optical biosensors. Furthermore, in comparison with other optical transducers that require complex nanofabrication techniques, electrospun nanofibers can be deposited over vast areas to create several transducers in a single batch and at a low cost.enginfo:eu-repo/semantics/restrictedAccessconference output10.1117/12.2576527Fabry-Pérot cavityLabel-free biosensingNanofibersOptical biosensorPorous materialsElectronic, Optical and Magnetic MaterialsCondensed Matter PhysicsComputer Science ApplicationsApplied MathematicsElectrical and Electronic EngineeringSDG 9 - Industry, Innovation, and Infrastructurehttp://www.scopus.com/inward/record.url?scp=85108460744&partnerID=8YFLogxK