Novel Concept of Micro Patterned Micro Titer Plates Fabricated via UV-NIL for Automated Neuronal Cell Assay Read-Out

Lohse, MirkoThesen, Manuel W.Haase, AnjaSmolka, MartinIceta, Nerea BrizAyerdi Izquierdo, AnaRamos, IsbaalSalado, ClarisaSchleunitz, Arne2021-04-01Lohse , M , Thesen , M W , Haase , A , Smolka , M , Iceta , N B , Ayerdi Izquierdo , A , Ramos , I , Salado , C & Schleunitz , A 2021 , ' Novel Concept of Micro Patterned Micro Titer Plates Fabricated via UV-NIL for Automated Neuronal Cell Assay Read-Out ' , Nanomaterials , vol. 11 , no. 4 , 902 , pp. 902 . https://doi.org/10.3390/nano110409022079-4991researchoutputwizard: 11556/1108Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.The UV-nanoimprint lithography(UV-NIL) fabrication of a novel network of micron-sized channels, forming an open channel microfluidic system is described. Details about the complete manufacturing process, from mastering to fabrication in small batches and in high throughput with up to 1200 micro titer plates per hour is presented. Deep insight into the evaluation of a suitable UV-curable material, mr-UVCur26SF is given, presenting cytotoxic evaluation, cell compatibility tests and finally a neuronal assay. The results indicate how the given pattern, in combination with the resist, paves the way to faster, cheaper, and more reliable drug screening.11196866enginfo:eu-repo/semantics/openAccessNovel Concept of Micro Patterned Micro Titer Plates Fabricated via UV-NIL for Automated Neuronal Cell Assay Read-Outjournal article10.3390/nano11040902Nanoimprint lithographyR2R UV-NILNeuronal cell assayNanoimprint lithographyR2R UV-NILNeuronal cell assayGeneral Chemical EngineeringGeneral Materials ScienceProject IDinfo:eu-repo/grantAgreement/EC/H2020/646260/EU/Large scale micro-and nanofabrication technologies for bioanalytical devices based on R2R imprinting/R2R Biofluidicsinfo:eu-repo/grantAgreement/EC/H2020/862092/EU/Next generation test bed for upscaling of microfluidic devices based on nano-enabled surfaces and membranes/NextGenMicrofluidicsinfo:eu-repo/grantAgreement/EC/H2020/646260/EU/Large scale micro-and nanofabrication technologies for bioanalytical devices based on R2R imprinting/R2R Biofluidicsinfo:eu-repo/grantAgreement/EC/H2020/862092/EU/Next generation test bed for upscaling of microfluidic devices based on nano-enabled surfaces and membranes/NextGenMicrofluidicsFunding InfoThis project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement NO. 646260. The research was also partially supported by NextGenMicrofluidics project under HORIZON2020 with grant agreement no 862092.This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement NO. 646260. The research was also partially supported by NextGenMicrofluidics project under HORIZON2020 with grant agreement no 862092.http://www.scopus.com/inward/record.url?scp=85103394776&partnerID=8YFLogxK