RT Journal Article T1 Production of Docosahexaenoic Acid and Odd-Chain Fatty Acids by Microalgae Schizochytrium limacinum Grown on Waste-Derived Volatile Fatty Acids A1 Oliver, Laura A1 Fernández-de-Castro, Laura A1 Dietrich, Thomas A1 Villaran, Maria Carmen A1 Barrio, Ramón J. AB Heterotrophic microalgae are recognized as a source of bioactive compounds. However, there are still some drawbacks for their use at an industrial scale associated with the high cost of glucose, the main carbon source in heterotrophic cultures. In recent years, significant efforts have been made to investigate more sustainable carbon sources to produce biomass. In this study, the capacity of Schizochytrium limacinum to grow on waste-derived volatile fatty acids and the effect that their use produces on biomass and fatty acids profiles were investigated. Acetic, propionic, butyric, valeric and caproic acid were evaluated independently, as well as in a synthetic mixture (VFA). The use of acetic and butyric resulted in a good biomass productivity, while the use of valeric and propionic acid resulted in higher content of odd-chain fatty acids (OCFA), increasingly investigated due to their potential benefits for human health. The use of industrial waste-derived VFA as a potential carbon source was validated through the utilization of biowaste derived effluents from a volatile fatty acid platform. The biomass produced was of 18.5 g/L, 54.0% lipids, 46.3% docosahexaenoic acid (DHA) and 25.0% OCFA, concluding that waste derived VFA can produce DHA and OCFA in a suitable ratio of DHA/OCFA with potential industrial applications. SN 2076-3417 YR 2022 FD 2022-04-14 LA eng NO Oliver , L , Fernández-de-Castro , L , Dietrich , T , Villaran , M C & Barrio , R J 2022 , ' Production of Docosahexaenoic Acid and Odd-Chain Fatty Acids by Microalgae Schizochytrium limacinum Grown on Waste-Derived Volatile Fatty Acids ' , Applied Sciences , vol. 12 , no. 8 , 3976 , pp. 3976 . https://doi.org/10.3390/app12083976 NO Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. DS TECNALIA Publications RD 3 jul 2024