RT Journal Article
T1 Enhancing Osteoconduction of PLLA-Based Nanocomposite Scaffolds for Bone Regeneration Using Different Biomimetic Signals to MSCs
A1 Ciapetti, Gabriela
A1 Granchi, Donatella
A1 Devescovi, Valentina
A1 Baglio, Serena R.
A1 Leonardi, Elisa
A1 Martini, Desirèe
A1 Jurado, Maria Jesus
A1 Olalde, Beatriz
A1 Armentano, Ilaria
A1 Kenny, Josè M.
A1 Walboomers, Frank X.
A1 Alava, J.I.
A1 Baldini, Nicola
AB In bone engineering, the adhesion, proliferation and differentiation of mesenchymal stromal cells rely on signaling from chemico-physical structure of the substrate, therefore prompting the design of mimetic “extracellular matrix”-like scaffolds. In this study, three-dimensional porous poly-L-lactic acid (PLLA)-based scaffolds have been mixed with different components, including single walled carbon nanotubes (CNT), micro-hydroxyapatite particles (HA), and BMP2, and treated with plasma (PT), to obtain four different nanocomposites: PLLA + CNT, PLLA + CNTHA, PLLA + CNT + HA + BMP2 and PLLA + CNT + HA + PT. Adult bone marrow mesenchymal stromal cells (MSCs) were derived from the femur of orthopaedic patients, seeded on the scaffolds and cultured under osteogenic induction up to differentiation and mineralization. The release of specific metabolites and temporal gene expression profiles of marrow-derived osteoprogenitors were analyzed at definite time points, relevant to in vitro culture as well asin vivo differentiation. As a result, the role of the different biomimetic components added to the PLLA matrix was deciphered, with BMP2-added scaffolds showing the highest biomimetic activity on cells differentiating to mature osteoblasts. The modification of a polymeric scaffold with reinforcing components which also work as biomimetic cues for cells can effectively direct osteoprogenitor cells differentiation, so as to shorten the time required for mineralization.
YR 2012
FD 2012-02
LA eng
NO Ciapetti , G , Granchi , D , Devescovi , V , Baglio , S R , Leonardi , E , Martini , D , Jurado , M J , Olalde , B , Armentano , I , Kenny , J M , Walboomers , F X , Alava , J I & Baldini , N 2012 , ' Enhancing Osteoconduction of PLLA-Based Nanocomposite Scaffolds for Bone Regeneration Using Different Biomimetic Signals to MSCs ' , unknown , vol. unknown , no. 2 , pp. 2439-2458 . https://doi.org/10.3390/ijms13022439
DS TECNALIA Publications
RD 1 jul 2024