RT Conference Proceedings
T1 Low-cost electrospun nanofibers for label-free and real-time optical biodetection purposes
A1 Martínez-Pérez, Paula
A1 Ponce-Alcántara, Salvador
A1 Murillo, Nieves
A1 Pérez-Márquez, Ana
A1 Maudes, Jon
A1 Peraile, Inés
A1 González-López, Laura
A1 Gil-García, Matilde
A1 Lorenzo-Lozano, Paloma
A1 García-Rupérez, Jaime
A2 Fixler, Dror
A2 Goldys, Ewa M.
A2 Wachsmann-Hogiu, Sebastian
AB In the development of label-free and real-time optical biosensors, a straightforward and low-cost fabrication of the transducer is important, because it reduces costs and the complexity of the process. One possibility for such easy fabrication is electrospinning. It is a versatile and well-developed technique that allows the manufacturing of layers of nanofibers at a low cost with a Fabry-Pérot cavity-based optical response when illuminated with visible light. In this research, the suitability of such layers for the development of label-free and real-time optical biosensors is studied. For such aim, the nanofibers were biofunctionalized in flow with antibodies against bovine serum albumin through an intermediate layer of protein A/G. Then, BSA was flowed at a concentration of 10 μg/ml as the target analyte. As a result, biofunctionalization and biodetection processes were optically monitored in real-time successfully, demonstrating the suitability of such a simple-to-fabricate transducer for the development of label-free and real-time optical biosensors. Furthermore, in comparison with other optical transducers that require complex nanofabrication techniques, electrospun nanofibers can be deposited over vast areas to create several transducers in a single batch and at a low cost.
PB SPIE
SN 9781510641518
SN 0277-786X
YR 2021
FD 2021
LA eng
NO Martínez-Pérez , P , Ponce-Alcántara , S , Murillo , N , Pérez-Márquez , A , Maudes , J , Peraile , I , González-López , L , Gil-García , M , Lorenzo-Lozano , P & García-Rupérez , J 2021 , Low-cost electrospun nanofibers for label-free and real-time optical biodetection purposes . in D Fixler , E M Goldys & S Wachsmann-Hogiu (eds) , Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVIII . , 116580E , Proceedings of SPIE - The International Society for Optical Engineering , vol. 11658 , SPIE , Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVIII 2021 , Vierual, Online , United States , 6/03/21 . https://doi.org/10.1117/12.2576527
NO conference
NO Publisher Copyright: © 2021 SPIE. All rights reserved.
NO This research was supported by a co-financed action by the European Union through the operational program of the European Regional Development Fund (FEDER) of the Valencian Community 2014–2020, the Spanish government through the projects TEC2015-63838-C3-OPTONANOSENS and PID2019-106965RB-C21-PHOLOW, the Generalitat Valenciana through the PROMETEO project AVANTI/2019/123 and the grant PPC/2020/037, the Basque government through the project μ4Industry, KK-2019/00101, from the ELKARTEK Program, and the Universitat Politècnica de València through grant PAID-01-17.
DS TECNALIA Publications
RD 30 jul 2024