RT Journal Article
T1 Cell Retention as a Viable Strategy for PHA Production from Diluted VFAs with Bacillus megaterium
A1 Kacanski, Milos
A1 Pucher, Lukas
A1 Peral, Carlota
A1 Dietrich, Thomas
A1 Neureiter, Markus
AB The production of biodegradable and biocompatible materials such as polyhydroxyalkanoates (PHAs) from waste-derived volatile fatty acids (VFAs) is a promising approach towards implementing a circular bioeconomy. However, VFA solutions obtained via acidification of organic wastes are usually too diluted for direct use in standard batch or fed-batch processes. To overcome these constraints, this study introduces a cell recycle fed-batch system using Bacillus megaterium uyuni S29 for poly(3-hydroxybutyrate) (P3HB) production from acetic acid. The concentrations of dry cell weight (DCW), P3HB, acetate, as well as nitrogen as the limiting substrate component, were monitored during the process. The produced polymer was characterized in terms of molecular weight and thermal properties after extraction with hypochlorite. The results show that an indirect pH-stat feeding regime successfully kept the strain fed without prompting inhibition, resulting in a dry cell weight concentration of up to 19.05 g/L containing 70.21% PHA. After appropriate adaptations the presented process could contribute to an efficient and sustainable production of biopolymers.
SN 2306-5354
YR 2022
FD 2022-03-16
LA eng
NO Kacanski , M , Pucher , L , Peral , C , Dietrich , T & Neureiter , M 2022 , ' Cell Retention as a Viable Strategy for PHA Production from Diluted VFAs with Bacillus megaterium ' , Bioengineering , vol. 9 , no. 3 , 122 , pp. 122 . https://doi.org/10.3390/bioengineering9030122
NO Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
DS TECNALIA Publications
RD 3 jul 2024