RT Journal Article
T1 MiR-219a-5p Enriched Extracellular Vesicles Induce OPC Differentiation and EAE Improvement More Efficiently Than Liposomes and Polymeric Nanoparticles
A1 Osorio-Querejeta, Iñaki
A1 Carregal-Romero, Susana
A1 Ayerdi-Izquierdo, Ana
A1 Mäger, Imre
A1 Nash, Leslie A.
A1 Wood, Matthew
A1 Egimendia, Ander
A1 Betanzos, M.
A1 Alberro, Ainhoa
A1 Iparraguirre, Leire
A1 Moles, Laura
A1 Llarena, Irantzu
A1 Möller, Marco
A1 Goñi-de-Cerio, Felipe
A1 Bijelic, Goran
A1 Ramos-Cabrer, Pedro
A1 Muñoz-Culla, Maider
A1 Otaegui, David
AB Remyelination is a key aspect in multiple sclerosis pathology and a special effort is being made to promote it. However, there is still no available treatment to regenerate myelin and several strategies are being scrutinized. Myelination is naturally performed by oligodendrocytes and microRNAs have been postulated as a promising tool to induce oligodendrocyte precursor cell differentiation and therefore remyelination. Herein, DSPC liposomes and PLGA nanoparticles were studied for miR-219a-5p encapsulation, release and remyelination promotion. In parallel, they were compared with biologically engineered extracellular vesicles overexpressing miR-219a-5p. Interestingly, extracellular vesicles showed the highest oligodendrocyte precursor cell differentiation levels and were more effective than liposomes and polymeric nanoparticles crossing the blood–brain barrier. Finally, extracellular vesicles were able to improve EAE animal model clinical evolution. Our results indicate that the use of extracellular vesicles as miR-219a-5p delivery system can be a feasible and promising strategy to induce remyelination in multiple sclerosis patients.
SN 1999-4923
YR 2020
FD 2020-02
LA eng
NO Osorio-Querejeta , I , Carregal-Romero , S , Ayerdi-Izquierdo , A , Mäger , I , Nash , L A , Wood , M , Egimendia , A , Betanzos , M , Alberro , A , Iparraguirre , L , Moles , L , Llarena , I , Möller , M , Goñi-de-Cerio , F , Bijelic , G , Ramos-Cabrer , P , Muñoz-Culla , M & Otaegui , D 2020 , ' MiR-219a-5p Enriched Extracellular Vesicles Induce OPC Differentiation and EAE Improvement More Efficiently Than Liposomes and Polymeric Nanoparticles ' , Pharmaceutics , vol. 12 , no. 2 , 186 , pp. 186 . https://doi.org/10.3390/pharmaceutics12020186
NO Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
DS TECNALIA Publications
RD 3 jul 2024