RT Journal Article
T1 Label-free optical biosensing using low-cost electrospun polymeric nanofibers
A1 Martínez-Pérez, Paula
A1 Ponce-Alcántara, Salvador
A1 Murillo, Nieves
A1 Pérez-Márquez, Ana
A1 Maudes, Jon
A1 Peraile, Inés
A1 González-López, Laura
A1 Gil-García, Matilde
A1 Lorenzo-Lozano, Paloma
A1 García-Rupérez, Jaime
AB Polymeric nanofiber matrices are promising structures to develop biosensing devices due to their easy and affordable large-scale fabrication and their high surface-to-volume ratio. In this work, the suitability of a polyamide 6 nanofiber matrix for the development of a label-free and real-time Fabry–Pérot cavity-based optical biosensor was studied. For such aim, in-flow biofunctionalization of nanofibers with antibodies, bound through a protein A/G layer, and specific biodetection of 10 µg/mL bovine serum albumin (BSA) were carried out. Both processes were successfully monitored via reflectivity measurements in real-time without labels and their reproducibility was demonstrated when different polymeric nanofiber matrices from the same electrospinning batch were employed as transducers. These results demonstrate not only the suitability of correctly biofunctionalized polyamide 6 nanofiber matrices to be employed for real-time and label-free specific biodetection purposes, but also the potential of electrospinning technique to create affordable and easy-to-fabricate at large scale optical transducers with a reproducible performance.
SN 2227-9040
YR 2020
FD 2020-11-26
LA eng
NO Martínez-Pérez , P , Ponce-Alcántara , S , Murillo , N , Pérez-Márquez , A , Maudes , J , Peraile , I , González-López , L , Gil-García , M , Lorenzo-Lozano , P & García-Rupérez , J 2020 , ' Label-free optical biosensing using low-cost electrospun polymeric nanofibers ' , Chemosensors , vol. 8 , no. 4 , 119 , pp. 1-11 . https://doi.org/10.3390/chemosensors8040119 , https://doi.org/10.3390/chemosensors8040119
NO Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
NO Funding: This research was supported by a co-financed action by the European Union through the operational program of the European Regional Development Fund (FEDER) of the Valencian Community 2014–2020, the Generalitat Valenciana through the PROMETEO project AVANTI/2019/123 and the grant PPC/2020/037, the Spanish government through the project TEC2015-63838-C3-OPTONANOSENS, Universitat Politècnica de València through grant PAID-01-17, and by the Basque government through the project µ4Industry, KK-2019/00101, from the ELKARTEK Program.
DS TECNALIA Publications
RD 3 jul 2024