RT Journal Article
T1 Role of PLLA plasma surface modification in the interaction with human marrow stromal cells
A1 Armentano, Ilaria
A1 Ciapetti, Gabriela
A1 Pennacchi, Manuela
A1 Dottori, Mariaserena
A1 Devescovi, Valentina
A1 Granchi, Donatella
A1 Baldini, Nicola
A1 Olalde, Beatriz
A1 Jurado, Maria Jesus
A1 Alava, Jose Inaki Marquinez
A1 Kenny, José M.
AB The effects of oxygen-based radio frequency plasma enhanced chemical vapor deposition (rf PECVD) on the surface of poly(L-lactide) (PLLA) polymers and the influence thereof on protein adsorption and on bone-cell behavior have been studied. Thin films and porous scaffolds based on PLLA polymer were developed, and the role of surface modifications were investigated extensively. PECVD surface treatments were used to alter surface functionality and modulate protein adsorption on the PLLA polymer matrix. In particular, Bovine Serum Albumine fluorescein isothiocyanate (fitc-BSA) conjugate adsorption on patterned surfaces of treated PLLA was analyzed by fluorescence microscopy. Human marrow stromal cells (MSCs) were cultured on scaffolds and cell adhesion and morphology were assessed using fluorescence microscopy. The results indicated that the PLLA surface became hydrophilic and its roughness increased with the treatment time and it had a dominant influence on the adsorption process of the protein. The outcome of the plasma treatment of various PLLA surfaces has been shown to be the up-regulator of the cell-adhesive proteins expression and consequently the improvement of cell adhesion and growth. Oxygen-treated PLLA promoted higher adhesion and proliferation of the MSCs in comparison to the untreated samples. It can be concluded that following plasma treatment, PLLA samples show enhanced affinity for osteoprogenitor cells.
SN 0021-8995
YR 2009
FD 2009-12-15
LK https://hdl.handle.net/11556/3717
UL https://hdl.handle.net/11556/3717
LA eng
NO Armentano , I , Ciapetti , G , Pennacchi , M , Dottori , M , Devescovi , V , Granchi , D , Baldini , N , Olalde , B , Jurado , M J , Alava , J I M & Kenny , J M 2009 , ' Role of PLLA plasma surface modification in the interaction with human marrow stromal cells ' , Journal of Applied Polymer Science , vol. 114 , no. 6 , pp. 3602-3611 . https://doi.org/10.1002/app.31008
DS TECNALIA Publications
RD 30 jul 2024