RT Conference Proceedings
T1 Toxicogenomics study of nanomaterials on the model organism zebrafish
A1 Rojo, I.
A1 Uriarte, M.
A1 Obieta, I.
A1 Bustero, I.
A1 Egizabal, A.
A1 Pardo, M. A.
A1 De Martínez Ilárduya, O.
AB We have adapted an "in vitro" toxicity test using zebrafish embryos for a toxicogenomics approach. Our goal is to develop a rapid toxicity assay for nanoparticles to be used in food and other applications. We incubated fertilized fish eggs for 48 h in the presence of a solution of purified MWCNT (1 ppb to 10 ppm) or with nanosilver solutions (1 ppb to 5 ppm). We could not detect effects on development or timing. We have used quantitative RT-PCR to analyze the expression patterns of Cyp1A1, Ahr2, Cpt1, iNOS, Ncf1, NudT1, c/EBP-a and MafT. These genes are involved in detoxification patterns, in the protection against oxidative stress or code for regulatory factors involved in the immune response. At these concentrations, nanosilver had clear effects on the expression of most of the genes in a dose dependant manner. Induction was maximum in the case of regulatory factors. On the contrary, changes in gene expression induced by MWCNTs were much lower, suggesting a reduced toxicity at the tested concentrations.
SN 1420063421
SN 9781420063424
SN 1420061836
SN 9781420061833
YR 2007
FD 2007
LK https://hdl.handle.net/11556/1582
UL https://hdl.handle.net/11556/1582
LA eng
NO Rojo , I , Uriarte , M , Obieta , I , Bustero , I , Egizabal , A , Pardo , M A & De Martínez Ilárduya , O 2007 , Toxicogenomics study of nanomaterials on the model organism zebrafish . in 2007 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2007, Technical Proceedings . 2007 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2007, Technical Proceedings , vol. 2 , pp. 655-658 , 2007 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2007 , Santa Clara, CA , United States , 20/05/07 .
NO conference
DS TECNALIA Publications
RD 29 jul 2024