RT Journal Article
T1 Interfacing reduced graphene oxide with an adipose-derived extracellular matrix as a regulating milieu for neural tissue engineering
A1 Barroca, Nathalie
A1 da Silva, Daniela M.
A1 Pinto, Susana C.
A1 Sousa, Joana P.M.
A1 Verstappen, Kest
A1 Klymov, Alexey
A1 Fernández-San-Argimiro, Francisco Javier
A1 Madarieta, Iratxe
A1 Murua, Olatz
A1 Olalde, Beatriz
A1 Papadimitriou, Lina
A1 Karali, Kanelina
A1 Mylonaki, Konstantina
A1 Stratakis, Emmanuel
A1 Ranella, Anthi
A1 Marques, Paula A.A.P.
AB Enthralling evidence of the potential of graphene-based materials for neural tissue engineering is motivating the development of scaffolds using various structures related to graphene such as graphene oxide (GO) or its reduced form. Here, we investigated a strategy based on reduced graphene oxide (rGO) combined with a decellularized extracellular matrix from adipose tissue (adECM), which is still unexplored for neural repair and regeneration. Scaffolds containing up to 50 wt% rGO relative to adECM were prepared by thermally induced phase separation assisted by carbodiimide (EDC) crosslinking. Using partially reduced GO enables fine-tuning of the structural interaction between rGO and adECM. As the concentration of rGO increased, non-covalent bonding gradually prevailed over EDC-induced covalent conjugation with the adECM. Edge-to-edge aggregation of rGO favours adECM to act as a biomolecular physical crosslinker to rGO, leading to the softening of the scaffolds. The unique biochemistry of adECM allows neural stem cells to adhere and grow. Importantly, high rGO concentrations directly control cell fate by inducing the differentiation of both NE-4C cells and embryonic neural progenitor cells into neurons. Furthermore, primary astrocyte fate is also modulated as increasing rGO boosts the expression of reactivity markers while unaltering the expression of scar-forming ones.
SN 2772-9508
YR 2023
FD 2023-05
LA eng
NO Barroca , N , da Silva , D M , Pinto , S C , Sousa , J P M , Verstappen , K , Klymov , A , Fernández-San-Argimiro , F J , Madarieta , I , Murua , O , Olalde , B , Papadimitriou , L , Karali , K , Mylonaki , K , Stratakis , E , Ranella , A & Marques , P A A P 2023 , ' Interfacing reduced graphene oxide with an adipose-derived extracellular matrix as a regulating milieu for neural tissue engineering ' , Biomaterials Advances , vol. 148 , 213351 . https://doi.org/10.1016/j.bioadv.2023.213351
NO Publisher Copyright: © 2023
NO This work was supported by the European Union 's Horizon 2020 research and innovation programme under grant agreement No 829060 and the Portuguese following funding: UIDB/00481/2020 and UIDP/00481/2020 - Fundação para a Ciência e a Tecnologia (FCT) and CENTRO-01-0145-FEDER-022083 - Centro Portugal Regional Operational Programme (Centro2020) under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund . J.S. thanks FCT for the Ph.D. grant SFRH/BD/144579/2019 .
DS TECNALIA Publications
RD 30 jul 2024