RT Journal Article
T1 Biological response of laser macrostructured and oxidized titanium alloy: 												An in vitro and in vivo study
A1 Paz, María Dolores
A1 Iñaki Álava, J.
A1 Goikoetxea, Leire
A1 Chiussi, Stefano
A1 Díaz-Güemes, Idoia
A1 Usón, Jesus
A1 Sánchez, Francisco
A1 León, Betty
AB Purpose: To assess both the in vitro and in vivo biological response of a laser modified surface in an integrated manner. A combined innovative approach applies lasers to macrostructure as well as to oxidize the surface of titanium alloy implants. Materials and methods: A Nd:YAG marking and ArF excimer lasers were used for macrostructuring and UV-oxidizing the surface of Ti6Al4V discs, respectively. Human fetal osteoblastic cell culture and a sheep tibia model were used to assess the cell response and the osseogeneration capability of as-machined, laser macrostructured and laser macrostructured and oxidized surfaces. Results: In vitro: Laser macrostructuration alone did not promote cell response. Cellular proliferation was enhanced by the additional UV laser oxidation. In vivo: A greater significant percentage of bone-implant contact was obtained for both laser treated surfaces compared to machine-turned control samples, three months after implantation, in spite of the low cellular response for macrostructured samples. The use of sheep model for six months appears to be less adequate for a comparison because of the high level of bone integration in all samples. In spite of the often reported positive effect of titanium oxidation on the triggering of faster osseointegration, in this experiment the additional UV laser oxidation did not lead to a significant in vivo improvement. Conclusions: Laser macrostructuration of titanium alloy surfaces appears to promote bone apposition and may therefore constitute a promising surface modification strategy. In animal models, the natural process of titanium surface oxidation, because of physiologic fluids, alters properties observed in vitro with cells.
SN 1722-6899
YR 2011
FD 2011-09
LK https://hdl.handle.net/11556/3169
UL https://hdl.handle.net/11556/3169
LA eng
NO Paz , M D , Iñaki Álava , J , Goikoetxea , L , Chiussi , S , Díaz-Güemes , I , Usón , J , Sánchez , F & León , B 2011 , ' Biological response of laser macrostructured and oxidized titanium alloy : An in vitro and in vivo study ' , Journal of Applied Biomaterials and Biomechanics , vol. 9 , no. 3 , pp. 214-222 . https://doi.org/10.5301/JABB.2011.8923
DS TECNALIA Publications
RD 30 jul 2024