RT Journal Article
T1 Biointegration of corneal macroporous membranes based on poly(ethyl acrylate) copolymers in an experimental animal model
A1 Del Barrio, Jorge L.Alió
A1 Chiesa, Massimo
A1 Ferrer, Gloria Gallego
A1 Garagorri, Nerea
A1 Briz, Nerea
A1 Fernandez-Delgado, Jorge
A1 Valls, Maria Sancho Tello
A1 Botella, Carmen Carda
A1 García-Tuñón, Ignacio
A1 Bataille, Laurent
A1 Rodriguez, Alejandra
A1 Arnalich-Montiel, Francisco
A1 Ribelles, Jose L.Gómez
A1 Antolinos-Turpín, Carmen M.
A1 Gómez-Tejedor, Jose A.
A1 Alió, Jorge L.
A1 De Miguel, Maria P.
AB Currently available keratoprosthesis models (non-biological corneal substitutes) have a less than 75% graft survival rate at 2 years. We aimed at developing a model for keratoprosthesis based on the use of poly(ethyl acrylate) (PEA)-based copolymers, extracellular matrix-protein coating and colonization with adipose-derived mesenchymal stem cells. Human adipose tissue derived mesenchymal stem cells (h-ADASC) colonization efficiency of seven PEA-based copolymers in combination with four extracellular matrix coatings were evaluated in vitro. Then, macroporous membranes composed of the optimal PEA subtypes and coating proteins were implanted inside rabbit cornea. After a 3-month follow-up, the animals were euthanized, and the clinical and histological biointegration of the implanted material were assessed. h-ADASC adhered and survived when cultured in all PEA-based macroporous membranes. The addition of high hydrophilicity to PEA membranes decreased h-ADASC colonization in vitro. PEA-based copolymer containing 10% hydroxyethyl acrylate (PEA-HEA10) or 10% acrylic acid (PEA-AAc10) monomeric units showed the best cellular colonization rates. Collagen plus keratan sulfate-coated polymers demonstrated enhanced cellular colonization respect to fibronectin, collagen, or uncoated PEAs. In vivo implantation of membranes resulted in an extrusion rate of 72% for PEA, 50% for PEA-AAc10, but remarkably of 0% for PEA-HEA10. h-ADASC survival was demonstrated in all the membranes after 3 months follow-up. A slight reduction in the extrusion rate of h-ADASC colonized materials was observed. No significant differences between the groups with and without h-ADASC were detected respect to transparency or neovascularization. We propose PEA with low hydroxylation as a scaffold for the anchoring ring of future keratoprosthesis.
SN 1549-3296
YR 2015
FD 2015-03-01
LA eng
NO Del Barrio , J L A , Chiesa , M , Ferrer , G G , Garagorri , N , Briz , N , Fernandez-Delgado , J , Valls , M S T , Botella , C C , García-Tuñón , I , Bataille , L , Rodriguez , A , Arnalich-Montiel , F , Ribelles , J L G , Antolinos-Turpín , C M , Gómez-Tejedor , J A , Alió , J L & De Miguel , M P 2015 , ' Biointegration of corneal macroporous membranes based on poly(ethyl acrylate) copolymers in an experimental animal model ' , Journal of Biomedical Materials Research - Part A , vol. 103 , no. 3 , pp. 1106-1118 . https://doi.org/10.1002/jbm.a.35249
NO Publisher Copyright: © 2014 Wiley Periodicals, Inc.
DS TECNALIA Publications
RD 3 jul 2024