RT Journal Article
T1 Protein hydrolysate from organic fraction of municipal solid waste compost as nitrogen source to produce lactic acid by Lactobacillus fermentum ATCC 9338 and Lactobacillus plantarum NCIMB 8826
A1 Izaguirre, Jon Kepa
A1 Dietrich, Thomas
A1 Villarán, M. Carmen
A1 Castañón, Sonia
AB In this work a strategy for obtaining free amino-acids concentrate from an organic fraction of municipal solid waste compost and its use as a nitrogen source for lactic acid production, a compound widely used in different industries, using L. fermentum ATCC 9338 and L. plantarum NCIMB 8826 strains is described. Enzymatic digestion is based on the combined action of endoprotease Alcalase 1.5 MG and exoprotease Flavourzyme 500 MG. The highest degree of hydrolysis obtained under the optimal conditions was 41%. The use of glucanase Viscozyme L prior to protein hydrolysis helped to reduce the viscosity of the solution and promote the action of proteases, increasing its hydrolysis degree by 76%. The hydrolysate contained all 21 amino-acids, making it ideal for lactic acid bacteria growth. During shake flask cultivations the culture media was complemented with glucose as carbon source. Finally, with the hydrolysate, a maximum lactic acid concentration of 9.0 ± 0.2 g·L−1 and 11.1 ± 0.1 g·L−1 for L. fermentum ATCC 9338 and L. plantarum NCIMB 8826 respectively was obtained after 27 h. The innovation of the approach lies in exploiting the overproduction of compost for the production of lactic acid.
SN 1359-5113
YR 2020
FD 2020-01
LK https://hdl.handle.net/11556/3292
UL https://hdl.handle.net/11556/3292
LA eng
NO Izaguirre , J K , Dietrich , T , Villarán , M C & Castañón , S 2020 , ' Protein hydrolysate from organic fraction of municipal solid waste compost as nitrogen source to produce lactic acid by Lactobacillus fermentum ATCC 9338 and Lactobacillus plantarum NCIMB 8826 ' , Process Biochemistry , vol. 88 , pp. 15-21 . https://doi.org/10.1016/j.procbio.2019.09.028
NO Publisher Copyright: © 2019
NO Jon Kepa Izaguirre would like to acknowledge the Basque Government for awarding a predoctoral fellowship to achieve his PhD degree in the context of this research project. The authors are grateful to Dr. L. M. Quiros from Oviedo University for donating the strains L . fermentum ATCC 9338 and L . plantarum NCIMB 8826.
DS TECNALIA Publications
RD 30 jul 2024