RT Journal Article
T1 Upgrading the organic fraction of municipal solid waste to poly(3-hydroxybutyrate)
A1 Izaguirre, Jon Kepa
A1 da Fonseca, M. Manuela R.
A1 Fernandes, Pedro
A1 Villarán, M. Carmen
A1 Castañón, Sonia
A1 Cesário, M. Teresa
AB The organic fraction of municipal solid waste was studied as feedstock for the production of poly(3-hydroxybutyrate) (P(3HB)). To release the monosaccharides, a diluted acid pre-treatment followed by an enzymatic hydrolysis was applied. A sugar yield of 49% was achieved using a pre-treated waste and an enzyme cocktail of Pentopan 500 BG and Celluclast BG. The addition of Glucoamylase NS 22035 helped to hydrolyze the starch fraction, improving the hydrolysis yield to 56%. The hydrolysate was used as culture medium to produce P(3HB) by Burkholderia sacchari DSM 17165. Assays at shaking flask scale showed that when the hydrolysate was used as substrate, the attained cell concentration was slightly higher than in the control medium. It was necessary to supplement the hydrolysate with extra glucose to increase the C/N ratio and with a mineral solution to overcome the nutritional deficiencies. The P(3HB) accumulation using the supplemented hydrolysate was 58% (g polymer/g biomass).
SN 0960-8524
YR 2019
FD 2019-10
LK https://hdl.handle.net/11556/3018
UL https://hdl.handle.net/11556/3018
LA eng
NO Izaguirre , J K , da Fonseca , M M R , Fernandes , P , Villarán , M C , Castañón , S & Cesário , M T 2019 , ' Upgrading the organic fraction of municipal solid waste to poly(3-hydroxybutyrate) ' , Bioresource Technology , vol. 290 , 121785 . https://doi.org/10.1016/j.biortech.2019.121785
NO Publisher Copyright: © 2019 Elsevier Ltd
NO The authors are thankful for the technical and human support provided by SGIker of UPV/EHU and for the European funding ( ERDF and ESF ). Funding received by iBB-Institute for Bioengineering and Biosciences from FCT -Portuguese Foundation for Science and Technology (grant UID/BIO/04565/2013 ) and from Programa Operacional Regional de Lisboa 2020 (Project N. 007317) is acknowledged. Jon Kepa Izaguirre would like to acknowledge the Basque Government for awarding a predoctoral fellowship to achieve his PhD degree in the context of this research project. The authors are thankful for the technical and human support provided by SGIker of UPV/EHU and for the European funding (ERDF and ESF). Funding received by iBB-Institute for Bioengineering and Biosciences from FCT-Portuguese Foundation for Science and Technology (grant UID/BIO/04565/2013) and from Programa Operacional Regional de Lisboa 2020 (Project N. 007317) is acknowledged. Jon Kepa Izaguirre would like to acknowledge the Basque Government for awarding a predoctoral fellowship to achieve his PhD degree in the context of this research project.
DS TECNALIA Publications
RD 30 jul 2024