RT Journal Article
T1 Influence of menopause on adipose tissue clock gene genotype and its relationship with metabolic syndrome in morbidly obese women
A1 Hernandez-Morante, Juan José
A1 Gomez-Santos, Cecilia
A1 Margareto, Javier
A1 Formiguera, Xavier
A1 Martínez, Carlos Manuel
A1 González, Raquel
A1 Martínez-Augustín, Olga
A1 Madrid, Juan Antonio
A1 Ordovas, Jose María
A1 Garaulet, Marta
AB Menopausal women exhibit a loss of circadian coordination, a process that runs parallel with a redistribution of adipose tissue. However, the specific genetic mechanisms underlying these alterations have not been studied. Thus, the aim of the present study was to determine whether the development of menopause induces an alteration of the genes that control biological rhythms in human subcutaneous (SAT) and visceral (VAT) adipose tissue, and whether changes in clock gene expression are involved in the increased risk of developing metabolic syndrome (MetS), which is frequently associated with menopause. To this end, VAT and SAT biopsies were taken in pre- (n = 7) and postmenopausal (n = 7) women at similar hours in the morning. RNA was extracted, and a microarray analysis was made. Data were confirmed by quantitative real-time polymerase chain reaction. Western blot and immunohistochemical analysis were also performed. When clock gene expression was compared between both groups of women, data in SAT showed that expression of the core clock gene period3 was significantly higher in postmenopausal women, while casein kinase-1δ, E1A-binding protein and cAMP-responsive element were preferentially expressed in the premenopausal group. In VAT, period2 (PER2) and v-myc myelocytomatosis viral oncogene expressions were significantly higher in the postmenopausal group. Western blot analysis indicated that PER2 and PER3 protein expression was also increased in postmenopausal women. In addition, several genes, including PER2, were differentially expressed depending on whether or not the patient met the MetS criteria. We conclude that menopause transition induces several changes in the genotype of the adipose tissue chronobiological machinery related to an increased risk of developing MetS.
SN 0161-9152
YR 2012
FD 2012-12
LK https://hdl.handle.net/11556/3888
UL https://hdl.handle.net/11556/3888
LA eng
NO Hernandez-Morante , J J , Gomez-Santos , C , Margareto , J , Formiguera , X , Martínez , C M , González , R , Martínez-Augustín , O , Madrid , J A , Ordovas , J M & Garaulet , M 2012 , ' Influence of menopause on adipose tissue clock gene genotype and its relationship with metabolic syndrome in morbidly obese women ' , Age , vol. 34 , no. 6 , pp. 1369-1380 . https://doi.org/10.1007/s11357-011-9309-2
DS TECNALIA Publications
RD 26 jul 2024