Production of PHB from Chicory Roots - Comparison of Three Cupriavidus necator Strains
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2015-06-03
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Abstract
Chicory roots from hydroponic salad cultivation are an abundant food residue in Navarra (Spain) that are underutilized to date. Aiming at a holistic utilization of resources, we report here the first process using chicory root hydrolysate for the production of poly([R]-3-hydroxybutyrate) (PHB). The polymer can be used for packaging material made for the locally produced vegetables. In the first step, we developed a pre-treatment process to obtain a hydrolysate, which contained 34 g L-1 sugars and 0.7 g L-1 total Kjeldahl nitrogen. This hydrolysate was used as fermentation substrate for three PHB-producing strains. Cupriavidus necator DSM 428 reached a dry biomass concentration of 11.3 g L-1 with a PHB content of 66 % in dry mass within 5 days. C. necator DSM 531 yielded 3.5 g L-1 dry biomass containing 46 % PHB within the same period. C. necator DSM 545 was superior over the other two in that 14.0 g L-1 of biomass containing 78 % PHB after only 3 days were obtained. These results show that even within the same species, the productivities on natural substrates are very different. The produced polymers were extracted using chloroform, and several thermo-physical parameters are in good accordance with published data. Overall, our holistic approach and the encouraging results prove that chicory roots are a viable fermentation substrate for PHB-production.
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Cichorium intybus , fructose , by-product , food residue , pre-treatment , hydrolysate , batch fermentation , Cupriavidus necator , P3HB; polymer characterisation , inulin , Cichorium intybus , fructose , by-product , food residue , pre-treatment , hydrolysate , batch fermentation , Cupriavidus necator , P3HB; polymer characterisation , inulin , P3HB , Polymer characterisation , Biochemistry , General Chemistry , Process Chemistry and Technology , Funding Info , This work was conducted as a part of the LEAD-ERA Project CARBIO, which was financed by the Basque Goverment and co-financed by the European Regional Development Fund (ERDF) of the European Union. , This work was conducted as a part of the LEAD-ERA Project CARBIO, which was financed by the Basque Goverment and co-financed by the European Regional Development Fund (ERDF) of the European Union.
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Haas , C , Steinwandter , V , Diaz De Apodaca , E , Maestro Madurga , B , Smerilli , M , Dietrich , T & Neureiter , M 2015 , ' Production of PHB from Chicory Roots - Comparison of Three Cupriavidus necator Strains ' , unknown , vol. unknown , no. 2 , pp. 99-112 . https://doi.org/10.15255/CABEQ.2014.2250