Enhancing Osteoconduction of PLLA-Based Nanocomposite Scaffolds for Bone Regeneration Using Different Biomimetic Signals to MSCs

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dc.contributor.authorCiapetti, Gabriela
dc.contributor.authorGranchi, Donatella
dc.contributor.authorDevescovi, Valentina
dc.contributor.authorBaglio, Serena R.
dc.contributor.authorLeonardi, Elisa
dc.contributor.authorMartini, Desirèe
dc.contributor.authorJurado, Maria Jesus
dc.contributor.authorOlalde, Beatriz
dc.contributor.authorArmentano, Ilaria
dc.contributor.authorKenny, Josè M.
dc.contributor.authorWalboomers, Frank X.
dc.contributor.authorAlava, J.I.
dc.contributor.authorBaldini, Nicola
dc.contributor.institutionTecnalia Research & Innovation
dc.contributor.institutionBiomateriales
dc.date.issued2012-02
dc.description.abstractIn bone engineering, the adhesion, proliferation and differentiation of mesenchymal stromal cells rely on signaling from chemico-physical structure of the substrate, therefore prompting the design of mimetic “extracellular matrix”-like scaffolds. In this study, three-dimensional porous poly-L-lactic acid (PLLA)-based scaffolds have been mixed with different components, including single walled carbon nanotubes (CNT), micro-hydroxyapatite particles (HA), and BMP2, and treated with plasma (PT), to obtain four different nanocomposites: PLLA + CNT, PLLA + CNTHA, PLLA + CNT + HA + BMP2 and PLLA + CNT + HA + PT. Adult bone marrow mesenchymal stromal cells (MSCs) were derived from the femur of orthopaedic patients, seeded on the scaffolds and cultured under osteogenic induction up to differentiation and mineralization. The release of specific metabolites and temporal gene expression profiles of marrow-derived osteoprogenitors were analyzed at definite time points, relevant to in vitro culture as well asin vivo differentiation. As a result, the role of the different biomimetic components added to the PLLA matrix was deciphered, with BMP2-added scaffolds showing the highest biomimetic activity on cells differentiating to mature osteoblasts. The modification of a polymeric scaffold with reinforcing components which also work as biomimetic cues for cells can effectively direct osteoprogenitor cells differentiation, so as to shorten the time required for mineralization.en
dc.description.statusPeer reviewed
dc.format.extent20
dc.format.extent649270
dc.identifier.citationCiapetti , G , Granchi , D , Devescovi , V , Baglio , S R , Leonardi , E , Martini , D , Jurado , M J , Olalde , B , Armentano , I , Kenny , J M , Walboomers , F X , Alava , J I & Baldini , N 2012 , ' Enhancing Osteoconduction of PLLA-Based Nanocomposite Scaffolds for Bone Regeneration Using Different Biomimetic Signals to MSCs ' , unknown , vol. unknown , no. 2 , pp. 2439-2458 . https://doi.org/10.3390/ijms13022439
dc.identifier.doi10.3390/ijms13022439
dc.identifier.otherresearchoutputwizard: 11556/23
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=84857665847&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofunknown
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subject.keywordsbone tissue engineering
dc.subject.keywordsbiomimetic nanocomposites
dc.subject.keywordsmesenchymal stem cell
dc.subject.keywordsbone tissue engineering
dc.subject.keywordsbiomimetic nanocomposites
dc.subject.keywordsmesenchymal stem cell
dc.subject.keywordsCatalysis
dc.subject.keywordsMolecular Biology
dc.subject.keywordsSpectroscopy
dc.subject.keywordsComputer Science Applications
dc.subject.keywordsPhysical and Theoretical Chemistry
dc.subject.keywordsOrganic Chemistry
dc.subject.keywordsInorganic Chemistry
dc.subject.keywordsFunding Info
dc.subject.keywordsEU STRP516943
dc.subject.keywordsEU STRP516943
dc.titleEnhancing Osteoconduction of PLLA-Based Nanocomposite Scaffolds for Bone Regeneration Using Different Biomimetic Signals to MSCsen
dc.typejournal article
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