Low-cost electrospun nanofibers for label-free and real-time optical biodetection purposes

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dc.contributor.authorMartínez-Pérez, Paula
dc.contributor.authorPonce-Alcántara, Salvador
dc.contributor.authorMurillo, Nieves
dc.contributor.authorPérez-Márquez, Ana
dc.contributor.authorMaudes, Jon
dc.contributor.authorPeraile, Inés
dc.contributor.authorGonzález-López, Laura
dc.contributor.authorGil-García, Matilde
dc.contributor.authorLorenzo-Lozano, Paloma
dc.contributor.authorGarcía-Rupérez, Jaime
dc.contributor.editorFixler, Dror
dc.contributor.editorGoldys, Ewa M.
dc.contributor.editorWachsmann-Hogiu, Sebastian
dc.contributor.institutionTecnalia Research & Innovation
dc.contributor.institutionPRINTEX
dc.date.issued2021
dc.descriptionPublisher Copyright: © 2021 SPIE. All rights reserved.
dc.description.abstractIn the development of label-free and real-time optical biosensors, a straightforward and low-cost fabrication of the transducer is important, because it reduces costs and the complexity of the process. One possibility for such easy fabrication is electrospinning. It is a versatile and well-developed technique that allows the manufacturing of layers of nanofibers at a low cost with a Fabry-Pérot cavity-based optical response when illuminated with visible light. In this research, the suitability of such layers for the development of label-free and real-time optical biosensors is studied. For such aim, the nanofibers were biofunctionalized in flow with antibodies against bovine serum albumin through an intermediate layer of protein A/G. Then, BSA was flowed at a concentration of 10 μg/ml as the target analyte. As a result, biofunctionalization and biodetection processes were optically monitored in real-time successfully, demonstrating the suitability of such a simple-to-fabricate transducer for the development of label-free and real-time optical biosensors. Furthermore, in comparison with other optical transducers that require complex nanofabrication techniques, electrospun nanofibers can be deposited over vast areas to create several transducers in a single batch and at a low cost.en
dc.description.sponsorshipThis research was supported by a co-financed action by the European Union through the operational program of the European Regional Development Fund (FEDER) of the Valencian Community 2014–2020, the Spanish government through the projects TEC2015-63838-C3-OPTONANOSENS and PID2019-106965RB-C21-PHOLOW, the Generalitat Valenciana through the PROMETEO project AVANTI/2019/123 and the grant PPC/2020/037, the Basque government through the project μ4Industry, KK-2019/00101, from the ELKARTEK Program, and the Universitat Politècnica de València through grant PAID-01-17.
dc.description.statusPeer reviewed
dc.identifier.citationMartínez-Pérez , P , Ponce-Alcántara , S , Murillo , N , Pérez-Márquez , A , Maudes , J , Peraile , I , González-López , L , Gil-García , M , Lorenzo-Lozano , P & García-Rupérez , J 2021 , Low-cost electrospun nanofibers for label-free and real-time optical biodetection purposes . in D Fixler , E M Goldys & S Wachsmann-Hogiu (eds) , Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVIII . , 116580E , Proceedings of SPIE - The International Society for Optical Engineering , vol. 11658 , SPIE , Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVIII 2021 , Vierual, Online , United States , 6/03/21 . https://doi.org/10.1117/12.2576527
dc.identifier.citationconference
dc.identifier.doi10.1117/12.2576527
dc.identifier.isbn9781510641518
dc.identifier.issn0277-786X
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85108460744&partnerID=8YFLogxK
dc.language.isoeng
dc.publisherSPIE
dc.relation.ispartofNanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVIII
dc.relation.ispartofseriesProceedings of SPIE - The International Society for Optical Engineering
dc.relation.projectIDEuropean Commission, EC
dc.relation.projectIDEusko Jaurlaritza, KK-2019/00101
dc.relation.projectIDGeneralitat Valenciana, GVA, AVANTI/2019/123-PPC/2020/037
dc.relation.projectIDUniversitat Politècnica de València, UPV, PAID-01-17
dc.relation.projectIDEuropean Regional Development Fund, ERDF, TEC2015-63838-C3-OPTONANOSENS-PID2019-106965RB-C21-PHOLOW
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subject.keywordsFabry-Pérot cavity
dc.subject.keywordsLabel-free biosensing
dc.subject.keywordsNanofibers
dc.subject.keywordsOptical biosensor
dc.subject.keywordsPorous materials
dc.subject.keywordsElectronic, Optical and Magnetic Materials
dc.subject.keywordsCondensed Matter Physics
dc.subject.keywordsComputer Science Applications
dc.subject.keywordsApplied Mathematics
dc.subject.keywordsElectrical and Electronic Engineering
dc.subject.keywordsSDG 9 - Industry, Innovation, and Infrastructure
dc.titleLow-cost electrospun nanofibers for label-free and real-time optical biodetection purposesen
dc.typeconference output
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