High yield production of monomer-free chitosan oligosaccharides by pepsin catalyzed hydrolysis of a high deacetylation degree chitosan

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dc.contributor.authorRoncal, Tomás
dc.contributor.authorOviedo, Alberto
dc.contributor.authorde Armentia, Iratxe López
dc.contributor.authorFernández, Laura
dc.contributor.authorVillarán, M. Carmen
dc.contributor.institutionBIOECONOMÍA Y CO2
dc.contributor.institutionAlimentación Sostenible
dc.date.issued2007-12-28
dc.description.abstractThe high molecular weight of chitosan, which results in a poor solubility at neutral pH values and high viscosity aqueous solutions, limits its potential uses in the fields of food, health and agriculture. However, most of these limitations are overcome by chitosan oligosaccharides obtained by enzymatic hydrolysis of the polymer. Several commercial enzymes with different original specificities were assayed for their ability to hydrolyze a 93% deacetylation degree chitosan and compared with a chitosanase. According to the patterns of viscosity decrease and reducing end formation, three enzymes-cellulase, pepsin and lipase A-were found to be particularly suitable for hydrolyzing chitosan at a level comparable to that achieved by chitosanase. Unlike the appreciable levels of both 2-amino-2-deoxy-d-glucose and 2-acetamido-2-deoxy-d-glucose monomers released from chitosan by the other enzymes after a 20 h-hydrolysis (4.6-9.1% of the total product weight), no monomer could be detected following pepsin cleavage. As a result, pepsin produced a higher yield of chitosan oligosaccharides than the other enzymes: 52% versus as much as 46%, respectively. Low molecular weight chitosans accounted for the remaining 48% of hydrolysis products. The calculated average polymerization degree of the products released by pepsin was around 16 units after 20 h of hydrolysis. This product pattern and yield are proposed to be related to the bond cleavage specificity of pepsin and the high deacetylation degree of chitosan used as substrate. The optimal reaction conditions for hydrolysis of chitosan by pepsin were 40 °C and pH 4.5, and an enzyme/substrate ratio of 1:100 (w/w) for reactions longer than 1 h.en
dc.description.sponsorshipWe thank the Spanish Ministry of Industry, Tourism and Trade for financial support of this project through the PROFIT program (FIT-060000-2005-362 and FIT-060000-2006-63). Funding to T.R. under the Torres Quevedo program of the Spanish Ministry of Education and Science and the European Social Fund is also acknowledged (PTQ2004-0945).
dc.description.statusPeer reviewed
dc.format.extent7
dc.identifier.citationRoncal , T , Oviedo , A , de Armentia , I L , Fernández , L & Villarán , M C 2007 , ' High yield production of monomer-free chitosan oligosaccharides by pepsin catalyzed hydrolysis of a high deacetylation degree chitosan ' , Carbohydrate Research , vol. 342 , no. 18 , pp. 2750-2756 . https://doi.org/10.1016/j.carres.2007.08.023
dc.identifier.doi10.1016/j.carres.2007.08.023
dc.identifier.issn0008-6215
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=36148960142&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofCarbohydrate Research
dc.relation.projectIDSpanish Ministry of Industry, Tourism and Trade, FIT-060000-2006-63-FIT-060000-2005-362
dc.relation.projectIDMinistério da Educação e Ciência, MEC
dc.relation.projectIDEuropean Social Fund, ESF, PTQ2004-0945
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subject.keywordsChitosan hydrolysis
dc.subject.keywordsChitosan oligosaccharides
dc.subject.keywordsd-Glucosamine
dc.subject.keywordsDeacetylation degree
dc.subject.keywordsN-Acetyl-d-glucosamine
dc.subject.keywordsPepsin
dc.subject.keywordsAnalytical Chemistry
dc.subject.keywordsBiochemistry
dc.subject.keywordsOrganic Chemistry
dc.titleHigh yield production of monomer-free chitosan oligosaccharides by pepsin catalyzed hydrolysis of a high deacetylation degree chitosanen
dc.typejournal article
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