Cell Retention as a Viable Strategy for PHA Production from Diluted VFAs with Bacillus megaterium

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dc.contributor.authorKacanski, Milos
dc.contributor.authorPucher, Lukas
dc.contributor.authorPeral, Carlota
dc.contributor.authorDietrich, Thomas
dc.contributor.authorNeureiter, Markus
dc.contributor.institutionTecnalia Research & Innovation
dc.contributor.institutionAlimentación Sostenible
dc.date.issued2022-03-16
dc.descriptionPublisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
dc.description.abstractThe production of biodegradable and biocompatible materials such as polyhydroxyalkanoates (PHAs) from waste-derived volatile fatty acids (VFAs) is a promising approach towards implementing a circular bioeconomy. However, VFA solutions obtained via acidification of organic wastes are usually too diluted for direct use in standard batch or fed-batch processes. To overcome these constraints, this study introduces a cell recycle fed-batch system using Bacillus megaterium uyuni S29 for poly(3-hydroxybutyrate) (P3HB) production from acetic acid. The concentrations of dry cell weight (DCW), P3HB, acetate, as well as nitrogen as the limiting substrate component, were monitored during the process. The produced polymer was characterized in terms of molecular weight and thermal properties after extraction with hypochlorite. The results show that an indirect pH-stat feeding regime successfully kept the strain fed without prompting inhibition, resulting in a dry cell weight concentration of up to 19.05 g/L containing 70.21% PHA. After appropriate adaptations the presented process could contribute to an efficient and sustainable production of biopolymers.en
dc.description.statusPeer reviewed
dc.format.extent1
dc.format.extent1606636
dc.identifier.citationKacanski , M , Pucher , L , Peral , C , Dietrich , T & Neureiter , M 2022 , ' Cell Retention as a Viable Strategy for PHA Production from Diluted VFAs with Bacillus megaterium ' , Bioengineering , vol. 9 , no. 3 , 122 , pp. 122 . https://doi.org/10.3390/bioengineering9030122
dc.identifier.doi10.3390/bioengineering9030122
dc.identifier.issn2306-5354
dc.identifier.otherresearchoutputwizard: 11556/1352
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85127719872&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofBioengineering
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subject.keywordsPolyhydroxyalkanoates
dc.subject.keywordsPoly(3-hydroxybutyrate)
dc.subject.keywordsCell retention
dc.subject.keywordsVolatile fatty acids
dc.subject.keywordsBacillus megaterium
dc.subject.keywordsPolyhydroxyalkanoates
dc.subject.keywordsPoly(3-hydroxybutyrate)
dc.subject.keywordsCell retention
dc.subject.keywordsVolatile fatty acids
dc.subject.keywordsBacillus megaterium
dc.subject.keywordsBioengineering
dc.subject.keywordsSDG 12 - Responsible Consumption and Production
dc.subject.keywordsProject ID
dc.subject.keywordsinfo:eu-repo/grantAgreement/EC/H2020/720777/EU/Biowaste-derived volatile fatty acid platform for biopolymers, bioactive compounds and chemical building blocks/Volatile
dc.subject.keywordsinfo:eu-repo/grantAgreement/EC/H2020/720777/EU/Biowaste-derived volatile fatty acid platform for biopolymers, bioactive compounds and chemical building blocks/Volatile
dc.subject.keywordsFunding Info
dc.subject.keywordsThis work was supported by the European project ‘Volatile—Biowaste-derived volatile fatty acid platform for biopolymers, bioactive compounds and chemical building blocks’ and has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement number 720777.
dc.subject.keywordsThis work was supported by the European project ‘Volatile—Biowaste-derived volatile fatty acid platform for biopolymers, bioactive compounds and chemical building blocks’ and has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement number 720777.
dc.titleCell Retention as a Viable Strategy for PHA Production from Diluted VFAs with Bacillus megateriumen
dc.typejournal article
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