Confocal and Histological Features After Poly(Ethylene Glycol) Diacrylate Corneal Inlay Implantation

Simple item page
dc.contributor.authorBidaguren, Aritz
dc.contributor.authorMendicute, Javier
dc.contributor.authorMadarieta, Iratxe
dc.contributor.authorGaragorri, Nerea
dc.contributor.institutionBiomateriales
dc.contributor.institutionTecnalia Research & Innovation
dc.date.issued2019-11
dc.descriptionPublisher Copyright: © 2019, Association for Research in Vision and Ophthalmology Inc.. All rights reserved.
dc.description.abstractPurpose: To evaluate the in vivo biocompatibility of photopolymerized poly(ethylene glycol) diacrylate (PEGDA) intrastromal inlays in rabbit corneas. Methods: Sixty-three eyes of 42 New Zealand rabbits were included. Manual intrastromal pockets were dissected in 42 eyes. PEGDA inlays were obtained using a specifically designed photomask and were inserted in the intrastromal pocket of 21 eyes (inlay group); the remaining 21 right eyes did not receive any implant (pocket-only group). Twenty-one eyes with no intervention were used as controls. In vivo confocal microscopy (IVCM) was performed at every visit. After 2 months, rabbits were sacrificed and corneas removed for histological analysis. Results: Corneas remained clear in all but two animals, and five cases of corneal neovascularization were seen (P = 0.2). Inlays remained stable without evidence of lateral or anterior migration, and no other complications were observed. No changes in anterior and posterior keratocyte density (P = 0.3 and P = 0.1, respectively) or endothelial cell density (P = 0.23) were observed between groups during the study time by IVCM. On pathology samples, thinning of the epithelium over the inlay area and epithelial hyperplasia over the edges were observed. A polygonal empty space with no evidence of PEGDA hydrogel within the midstroma was seen in the inlay group. Keratocytes were normal in shape and number in the vicinity of the PEGDA implant area. Conclusions: Photopolymerized PEGDA intrastromal inlays have shown relatively good safety and stability in rabbit corneas. Inlays were biostable in the corneal environment and remained transparent during follow up. Translational Relevance: The investigated PEGDA is promising for the development of biocompatible intrastromal implants.en
dc.description.statusPeer reviewed
dc.format.extent1
dc.format.extent799116
dc.identifier.citationBidaguren , A , Mendicute , J , Madarieta , I & Garagorri , N 2019 , ' Confocal and Histological Features After Poly(Ethylene Glycol) Diacrylate Corneal Inlay Implantation ' , Translational Vision Science & Technology , vol. 8 , no. 6 , 39 , pp. 39 . https://doi.org/10.1167/tvst.8.6.39
dc.identifier.doi10.1167/tvst.8.6.39
dc.identifier.issn2164-2591
dc.identifier.otherresearchoutputwizard: 11556/868
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85078097108&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofTranslational Vision Science & Technology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subject.keywordsConfocal microscopy
dc.subject.keywordsPoly(ethylene glycol) diacrylate
dc.subject.keywordsHydrogel
dc.subject.keywordsIntracorneal inlay
dc.subject.keywordsCorneal stroma
dc.subject.keywordsConfocal microscopy
dc.subject.keywordsPoly(ethylene glycol) diacrylate
dc.subject.keywordsHydrogel
dc.subject.keywordsIntracorneal inlay
dc.subject.keywordsCorneal stroma
dc.subject.keywordsBiomedical Engineering
dc.subject.keywordsOphthalmology
dc.subject.keywordsFunding Info
dc.subject.keywordsSupported in part by the Spanish Ministry of_x000D_ Science and Education through Apply Research, grant_x000D_ MAT2006-13708-CO2-01. The authors alone are_x000D_ responsible for the content and writing of the paper._x000D_ Disclosure: A. Bidaguren, None; J. Mendicute,_x000D_ None; I. Madarieta, Tecnalia (E); N. Garagorri,_x000D_ Tecnalia (E)
dc.subject.keywordsSupported in part by the Spanish Ministry of_x000D_ Science and Education through Apply Research, grant_x000D_ MAT2006-13708-CO2-01. The authors alone are_x000D_ responsible for the content and writing of the paper._x000D_ Disclosure: A. Bidaguren, None; J. Mendicute,_x000D_ None; I. Madarieta, Tecnalia (E); N. Garagorri,_x000D_ Tecnalia (E)
dc.titleConfocal and Histological Features After Poly(Ethylene Glycol) Diacrylate Corneal Inlay Implantationen
dc.typejournal article
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
i2164-2591-8-6-39 (1).pdf
Size:
780.39 KB
Format:
Adobe Portable Document Format
Download