Kinetic properties of soluble and immobilized Candida rugosa lipase

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dc.contributor.authorVirto, Mar'ia D.
dc.contributor.authorAgud, ISabel
dc.contributor.authorMontero, Sol
dc.contributor.authorBlanco, Alicia
dc.contributor.authorSolozabal, Rodolfo
dc.contributor.authorLascaray, Jos'e M.
dc.contributor.authorLlama, Mar'ia J.
dc.contributor.authorSerra, Juan L.
dc.contributor.authorLandeta, L. Carlos
dc.contributor.authorDe Renobales, Mertxe
dc.contributor.institutionCentros PRE-FUSION TECNALIA - (FORMER)
dc.contributor.institutionTecnalia Research & Innovation
dc.date.accessioned2024-07-24T12:10:30Z
dc.date.available2024-07-24T12:10:30Z
dc.date.issued1995-02
dc.description.abstractImmobilized lipase (triacylglycerol ester hydrolase, EC 3.1.1.3) from Candida rugosa has been immobilized on commercially available microporous polypropylene and used for the batch hydrolysis of different animal fats. The effect of the reaction products at concentrations similar to those obtained at 90% hydrolysis, both on soluble and immobilized lipase, was studied. Glycerol showed low inhibitory effect but oleic acid caused 50% inhibition. A mixture of free fatty acids present in the complete hydrolysis of beef tallow inhibited lipase activity more than 70%. The stability of the enzyme (both soluble and immobilized) was highest in the presence of 20% isooctane. The apparent Michaelis constant for each substrate for the soluble enzyme did not change on immobilization.en
dc.description.statusPeer reviewed
dc.format.extent10
dc.identifier.citationVirto , M D , Agud , IS , Montero , S , Blanco , A , Solozabal , R , Lascaray , J M , Llama , M J , Serra , J L , Landeta , L C & De Renobales , M 1995 , ' Kinetic properties of soluble and immobilized Candida rugosa lipase ' , Applied Biochemistry and Biotechnology , vol. 50 , no. 2 , pp. 127-136 . https://doi.org/10.1007/BF02783449
dc.identifier.doi10.1007/BF02783449
dc.identifier.issn0273-2289
dc.identifier.urihttps://hdl.handle.net/11556/4083
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=51249166693&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofApplied Biochemistry and Biotechnology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subject.keywordsanimal fats hydrolysis
dc.subject.keywordsCandida rugosa
dc.subject.keywordsEC 3.1.1.3
dc.subject.keywordsimmobilized enzyme
dc.subject.keywordskinetic parameters
dc.subject.keywordsLipase
dc.subject.keywordsorganic solvents
dc.subject.keywordsBiotechnology
dc.subject.keywordsBioengineering
dc.subject.keywordsBiochemistry
dc.subject.keywordsApplied Microbiology and Biotechnology
dc.subject.keywordsMolecular Biology
dc.titleKinetic properties of soluble and immobilized Candida rugosa lipaseen
dc.typejournal article
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