Determination of methylarginines in human plasma by HPLC with pre-column derivatization using naphthalenedicarboxaldehyde as fluorogenic agent
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2002-07
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A method has been developed for the determination of the main methylated L-arginines, NG-monomethyl-L-arginine (L-NMMA), NG,NG-dimethyl-L-arginine (asymmetric dimethylarginine, L-ADMA) in biological samples. The assay involves precolumn derivatization of methylarginines with naphthalenedicarboxaldehyde and cyanide followed by HPLC with fluorescence detection. The separation of derivatized methylated arginines on a reversed-phase column was achieved in less than 25 min. The SPE procedure developed, based on a cation-exchange resin, permits quantitative determination of analytes at concentrations as low as 0.54, 3.42, and 0.75 μg L-1, respectively, i.e. lower than their plasma levels. Recoveries in plasma were over 81% and the inter- and intra-day relative standard deviation values as measures of repeatability were less than 4.5%. This novel and rapid method can be employed in a routine clinical setting.
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Unceta , N , San Vicente , A , Goicolea , M A , Sallés , J & Barrio , R J 2002 , ' Determination of methylarginines in human plasma by HPLC with pre-column derivatization using naphthalenedicarboxaldehyde as fluorogenic agent ' , Journal of Separation Science , vol. 25 , no. 10-11 , pp. 665-670 . https://doi.org/10.1002/1615-9314(20020701)25:10/11<665::AID-JSSC665>3.0.CO;2-H