Role of PLLA plasma surface modification in the interaction with human marrow stromal cells

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dc.contributor.authorArmentano, Ilaria
dc.contributor.authorCiapetti, Gabriela
dc.contributor.authorPennacchi, Manuela
dc.contributor.authorDottori, Mariaserena
dc.contributor.authorDevescovi, Valentina
dc.contributor.authorGranchi, Donatella
dc.contributor.authorBaldini, Nicola
dc.contributor.authorOlalde, Beatriz
dc.contributor.authorJurado, Maria Jesus
dc.contributor.authorAlava, Jose Inaki Marquinez
dc.contributor.authorKenny, José M.
dc.contributor.institutionBiomateriales
dc.contributor.institutionTecnalia Research & Innovation
dc.date.accessioned2024-07-24T12:06:59Z
dc.date.available2024-07-24T12:06:59Z
dc.date.issued2009-12-15
dc.description.abstractThe effects of oxygen-based radio frequency plasma enhanced chemical vapor deposition (rf PECVD) on the surface of poly(L-lactide) (PLLA) polymers and the influence thereof on protein adsorption and on bone-cell behavior have been studied. Thin films and porous scaffolds based on PLLA polymer were developed, and the role of surface modifications were investigated extensively. PECVD surface treatments were used to alter surface functionality and modulate protein adsorption on the PLLA polymer matrix. In particular, Bovine Serum Albumine fluorescein isothiocyanate (fitc-BSA) conjugate adsorption on patterned surfaces of treated PLLA was analyzed by fluorescence microscopy. Human marrow stromal cells (MSCs) were cultured on scaffolds and cell adhesion and morphology were assessed using fluorescence microscopy. The results indicated that the PLLA surface became hydrophilic and its roughness increased with the treatment time and it had a dominant influence on the adsorption process of the protein. The outcome of the plasma treatment of various PLLA surfaces has been shown to be the up-regulator of the cell-adhesive proteins expression and consequently the improvement of cell adhesion and growth. Oxygen-treated PLLA promoted higher adhesion and proliferation of the MSCs in comparison to the untreated samples. It can be concluded that following plasma treatment, PLLA samples show enhanced affinity for osteoprogenitor cells.en
dc.description.statusPeer reviewed
dc.format.extent10
dc.identifier.citationArmentano , I , Ciapetti , G , Pennacchi , M , Dottori , M , Devescovi , V , Granchi , D , Baldini , N , Olalde , B , Jurado , M J , Alava , J I M & Kenny , J M 2009 , ' Role of PLLA plasma surface modification in the interaction with human marrow stromal cells ' , Journal of Applied Polymer Science , vol. 114 , no. 6 , pp. 3602-3611 . https://doi.org/10.1002/app.31008
dc.identifier.doi10.1002/app.31008
dc.identifier.issn0021-8995
dc.identifier.urihttps://hdl.handle.net/11556/3717
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=70349876299&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofJournal of Applied Polymer Science
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subject.keywordsBiocompatibility
dc.subject.keywordsBiodegradable
dc.subject.keywordsBiological applications of polymers
dc.subject.keywordsCold plasma
dc.subject.keywordsSurfaces
dc.subject.keywordsGeneral Chemistry
dc.subject.keywordsSurfaces, Coatings and Films
dc.subject.keywordsPolymers and Plastics
dc.subject.keywordsMaterials Chemistry
dc.titleRole of PLLA plasma surface modification in the interaction with human marrow stromal cellsen
dc.typejournal article
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