Toxicogenomics study of nanomaterials on the model organism zebrafish

Simple item page
dc.contributor.authorRojo, I.
dc.contributor.authorUriarte, M.
dc.contributor.authorObieta, I.
dc.contributor.authorBustero, I.
dc.contributor.authorEgizabal, A.
dc.contributor.authorPardo, M. A.
dc.contributor.authorDe Martínez Ilárduya, O.
dc.contributor.institutionTecnalia Research & Innovation
dc.contributor.institutionPRINTEX
dc.contributor.institutionBiomateriales
dc.date.accessioned2024-07-24T11:46:39Z
dc.date.available2024-07-24T11:46:39Z
dc.date.issued2007
dc.description.abstractWe have adapted an "in vitro" toxicity test using zebrafish embryos for a toxicogenomics approach. Our goal is to develop a rapid toxicity assay for nanoparticles to be used in food and other applications. We incubated fertilized fish eggs for 48 h in the presence of a solution of purified MWCNT (1 ppb to 10 ppm) or with nanosilver solutions (1 ppb to 5 ppm). We could not detect effects on development or timing. We have used quantitative RT-PCR to analyze the expression patterns of Cyp1A1, Ahr2, Cpt1, iNOS, Ncf1, NudT1, c/EBP-a and MafT. These genes are involved in detoxification patterns, in the protection against oxidative stress or code for regulatory factors involved in the immune response. At these concentrations, nanosilver had clear effects on the expression of most of the genes in a dose dependant manner. Induction was maximum in the case of regulatory factors. On the contrary, changes in gene expression induced by MWCNTs were much lower, suggesting a reduced toxicity at the tested concentrations.en
dc.description.statusPeer reviewed
dc.format.extent4
dc.identifier.citationRojo , I , Uriarte , M , Obieta , I , Bustero , I , Egizabal , A , Pardo , M A & De Martínez Ilárduya , O 2007 , Toxicogenomics study of nanomaterials on the model organism zebrafish . in 2007 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2007, Technical Proceedings . 2007 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2007, Technical Proceedings , vol. 2 , pp. 655-658 , 2007 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2007 , Santa Clara, CA , United States , 20/05/07 .
dc.identifier.citationconference
dc.identifier.isbn1420063421
dc.identifier.isbn9781420063424
dc.identifier.isbn1420061836
dc.identifier.isbn9781420061833
dc.identifier.urihttps://hdl.handle.net/11556/1582
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=34547964782&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartof2007 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2007, Technical Proceedings
dc.relation.ispartofseries2007 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2007, Technical Proceedings
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subject.keywordsMWCNT
dc.subject.keywordsNanosilver
dc.subject.keywordsReal-time PCR
dc.subject.keywordsToxicogenomics
dc.subject.keywordsZebrafish
dc.subject.keywordsMechanical Engineering
dc.titleToxicogenomics study of nanomaterials on the model organism zebrafishen
dc.typeconference output
Files