Interfacing reduced graphene oxide with an adipose-derived extracellular matrix as a regulating milieu for neural tissue engineering

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dc.contributor.authorBarroca, Nathalie
dc.contributor.authorda Silva, Daniela M.
dc.contributor.authorPinto, Susana C.
dc.contributor.authorSousa, Joana P.M.
dc.contributor.authorVerstappen, Kest
dc.contributor.authorKlymov, Alexey
dc.contributor.authorFernández-San-Argimiro, Francisco Javier
dc.contributor.authorMadarieta, Iratxe
dc.contributor.authorMurua, Olatz
dc.contributor.authorOlalde, Beatriz
dc.contributor.authorPapadimitriou, Lina
dc.contributor.authorKarali, Kanelina
dc.contributor.authorMylonaki, Konstantina
dc.contributor.authorStratakis, Emmanuel
dc.contributor.authorRanella, Anthi
dc.contributor.authorMarques, Paula A.A.P.
dc.contributor.institutionBiomateriales
dc.contributor.institutionSG
dc.date.issued2023-05
dc.descriptionPublisher Copyright: © 2023
dc.description.abstractEnthralling evidence of the potential of graphene-based materials for neural tissue engineering is motivating the development of scaffolds using various structures related to graphene such as graphene oxide (GO) or its reduced form. Here, we investigated a strategy based on reduced graphene oxide (rGO) combined with a decellularized extracellular matrix from adipose tissue (adECM), which is still unexplored for neural repair and regeneration. Scaffolds containing up to 50 wt% rGO relative to adECM were prepared by thermally induced phase separation assisted by carbodiimide (EDC) crosslinking. Using partially reduced GO enables fine-tuning of the structural interaction between rGO and adECM. As the concentration of rGO increased, non-covalent bonding gradually prevailed over EDC-induced covalent conjugation with the adECM. Edge-to-edge aggregation of rGO favours adECM to act as a biomolecular physical crosslinker to rGO, leading to the softening of the scaffolds. The unique biochemistry of adECM allows neural stem cells to adhere and grow. Importantly, high rGO concentrations directly control cell fate by inducing the differentiation of both NE-4C cells and embryonic neural progenitor cells into neurons. Furthermore, primary astrocyte fate is also modulated as increasing rGO boosts the expression of reactivity markers while unaltering the expression of scar-forming ones.en
dc.description.sponsorshipThis work was supported by the European Union 's Horizon 2020 research and innovation programme under grant agreement No 829060 and the Portuguese following funding: UIDB/00481/2020 and UIDP/00481/2020 - Fundação para a Ciência e a Tecnologia (FCT) and CENTRO-01-0145-FEDER-022083 - Centro Portugal Regional Operational Programme (Centro2020) under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund . J.S. thanks FCT for the Ph.D. grant SFRH/BD/144579/2019 .
dc.description.statusPeer reviewed
dc.identifier.citationBarroca , N , da Silva , D M , Pinto , S C , Sousa , J P M , Verstappen , K , Klymov , A , Fernández-San-Argimiro , F J , Madarieta , I , Murua , O , Olalde , B , Papadimitriou , L , Karali , K , Mylonaki , K , Stratakis , E , Ranella , A & Marques , P A A P 2023 , ' Interfacing reduced graphene oxide with an adipose-derived extracellular matrix as a regulating milieu for neural tissue engineering ' , Biomaterials Advances , vol. 148 , 213351 . https://doi.org/10.1016/j.bioadv.2023.213351
dc.identifier.doi10.1016/j.bioadv.2023.213351
dc.identifier.issn2772-9508
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85149058015&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofBiomaterials Advances
dc.relation.projectIDCentro Portugal Regional Operational Programme
dc.relation.projectIDCentro2020
dc.relation.projectIDFundação para a Ciência e a Tecnologia, FCT, CENTRO-01-0145-FEDER-022083
dc.relation.projectIDHorizon 2020, 829060-UIDP/00481/2020
dc.relation.projectIDEuropean Regional Development Fund, ERDF, SFRH/BD/144579/2019
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subject.keywordsAstrocytes reactivity
dc.subject.keywordsDecellularized extracellular matrix
dc.subject.keywordsNeural stem cells
dc.subject.keywordsNeural tissue engineering
dc.subject.keywordsReduced graphene oxide
dc.subject.keywordsBioengineering
dc.subject.keywordsBiomaterials
dc.subject.keywordsBiomedical Engineering
dc.titleInterfacing reduced graphene oxide with an adipose-derived extracellular matrix as a regulating milieu for neural tissue engineeringen
dc.typejournal article
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