Biological response of laser macrostructured and oxidized titanium alloy: An in vitro and in vivo study

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dc.contributor.authorPaz, María Dolores
dc.contributor.authorIñaki Álava, J.
dc.contributor.authorGoikoetxea, Leire
dc.contributor.authorChiussi, Stefano
dc.contributor.authorDíaz-Güemes, Idoia
dc.contributor.authorUsón, Jesus
dc.contributor.authorSánchez, Francisco
dc.contributor.authorLeón, Betty
dc.contributor.institutionBiomateriales
dc.date.accessioned2024-07-24T12:01:40Z
dc.date.available2024-07-24T12:01:40Z
dc.date.issued2011-09
dc.description.abstractPurpose: To assess both the in vitro and in vivo biological response of a laser modified surface in an integrated manner. A combined innovative approach applies lasers to macrostructure as well as to oxidize the surface of titanium alloy implants. Materials and methods: A Nd:YAG marking and ArF excimer lasers were used for macrostructuring and UV-oxidizing the surface of Ti6Al4V discs, respectively. Human fetal osteoblastic cell culture and a sheep tibia model were used to assess the cell response and the osseogeneration capability of as-machined, laser macrostructured and laser macrostructured and oxidized surfaces. Results: In vitro: Laser macrostructuration alone did not promote cell response. Cellular proliferation was enhanced by the additional UV laser oxidation. In vivo: A greater significant percentage of bone-implant contact was obtained for both laser treated surfaces compared to machine-turned control samples, three months after implantation, in spite of the low cellular response for macrostructured samples. The use of sheep model for six months appears to be less adequate for a comparison because of the high level of bone integration in all samples. In spite of the often reported positive effect of titanium oxidation on the triggering of faster osseointegration, in this experiment the additional UV laser oxidation did not lead to a significant in vivo improvement. Conclusions: Laser macrostructuration of titanium alloy surfaces appears to promote bone apposition and may therefore constitute a promising surface modification strategy. In animal models, the natural process of titanium surface oxidation, because of physiologic fluids, alters properties observed in vitro with cells.en
dc.description.statusPeer reviewed
dc.format.extent9
dc.identifier.citationPaz , M D , Iñaki Álava , J , Goikoetxea , L , Chiussi , S , Díaz-Güemes , I , Usón , J , Sánchez , F & León , B 2011 , ' Biological response of laser macrostructured and oxidized titanium alloy : An in vitro and in vivo study ' , Journal of Applied Biomaterials and Biomechanics , vol. 9 , no. 3 , pp. 214-222 . https://doi.org/10.5301/JABB.2011.8923
dc.identifier.doi10.5301/JABB.2011.8923
dc.identifier.issn1722-6899
dc.identifier.urihttps://hdl.handle.net/11556/3169
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=84855984323&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofJournal of Applied Biomaterials and Biomechanics
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subject.keywordsCell proliferation
dc.subject.keywordsDental implants
dc.subject.keywordsHistomorphometry
dc.subject.keywordsLaser surface treatments
dc.subject.keywordsOsseointegration
dc.subject.keywordsTitanium oxide layers
dc.subject.keywordsBiophysics
dc.subject.keywordsBioengineering
dc.subject.keywordsBiomaterials
dc.subject.keywordsBiomedical Engineering
dc.titleBiological response of laser macrostructured and oxidized titanium alloy: An in vitro and in vivo studyen
dc.typejournal article
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