Hydrolysis of animall fats by lipase at temperatures below their melting points
dc.contributor.author | de Renobales, Mertxe | |
dc.contributor.author | Agud, I. | |
dc.contributor.author | Lascaray, J. M. | |
dc.contributor.author | Múgica, J. C. | |
dc.contributor.author | Landeta, L. C. | |
dc.contributor.author | Solozábal, R. | |
dc.contributor.institution | Centros PRE-FUSION TECNALIA - (FORMER) | |
dc.contributor.institution | Tecnalia Research & Innovation | |
dc.date.accessioned | 2024-07-24T12:10:24Z | |
dc.date.available | 2024-07-24T12:10:24Z | |
dc.date.issued | 1992-08 | |
dc.description.abstract | We have studied the hydrolysis of high melting animal fats by the lipase from Candidarugosa at temperatures between 20°C and 37°C without the addition of surfactants or organic solvents. To establish the practical applications of this process we investigated the optimal conditions of the reaction at high substrate concentrations (50% fat w/v) to achieve 95% hydrolysis (or better) in 24 hours. Experiments were conducted in solid emulsions without constant stirring (500 ml total reaction volume). Under all conditions tested, edible pork lard was a better substrate than inedible beef tallow yielding up to 96% hydrolysis with as low as 0.3 g lipase/Kg fat or 98% hydrolysis with 0.5 g lipase/Kg fat. The optimum temperature for the hydrolysis of edible pork lard was around 30°C. Inedible beef tallow and pork lard did not exhibit a clear optimum temperature. Inedible lard gave results intermediate between those of edible lard and inedible beef tallow. | en |
dc.description.status | Peer reviewed | |
dc.format.extent | 6 | |
dc.identifier.citation | de Renobales , M , Agud , I , Lascaray , J M , Múgica , J C , Landeta , L C & Solozábal , R 1992 , ' Hydrolysis of animall fats by lipase at temperatures below their melting points ' , Biotechnology Letters , vol. 14 , no. 8 , pp. 683-688 . https://doi.org/10.1007/BF01021643 | |
dc.identifier.doi | 10.1007/BF01021643 | |
dc.identifier.issn | 0141-5492 | |
dc.identifier.uri | https://hdl.handle.net/11556/4073 | |
dc.identifier.url | http://www.scopus.com/inward/record.url?scp=0026701182&partnerID=8YFLogxK | |
dc.language.iso | eng | |
dc.relation.ispartof | Biotechnology Letters | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.subject.keywords | Biotechnology | |
dc.subject.keywords | Bioengineering | |
dc.subject.keywords | Applied Microbiology and Biotechnology | |
dc.title | Hydrolysis of animall fats by lipase at temperatures below their melting points | en |
dc.type | journal article |