Biointegration of corneal macroporous membranes based on poly(ethyl acrylate) copolymers in an experimental animal model

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dc.contributor.authorDel Barrio, Jorge L.Alió
dc.contributor.authorChiesa, Massimo
dc.contributor.authorFerrer, Gloria Gallego
dc.contributor.authorGaragorri, Nerea
dc.contributor.authorBriz, Nerea
dc.contributor.authorFernandez-Delgado, Jorge
dc.contributor.authorValls, Maria Sancho Tello
dc.contributor.authorBotella, Carmen Carda
dc.contributor.authorGarcía-Tuñón, Ignacio
dc.contributor.authorBataille, Laurent
dc.contributor.authorRodriguez, Alejandra
dc.contributor.authorArnalich-Montiel, Francisco
dc.contributor.authorRibelles, Jose L.Gómez
dc.contributor.authorAntolinos-Turpín, Carmen M.
dc.contributor.authorGómez-Tejedor, Jose A.
dc.contributor.authorAlió, Jorge L.
dc.contributor.authorDe Miguel, Maria P.
dc.contributor.institutionTecnalia Research & Innovation
dc.contributor.institutionSG
dc.date.issued2015-03-01
dc.descriptionPublisher Copyright: © 2014 Wiley Periodicals, Inc.
dc.description.abstractCurrently available keratoprosthesis models (non-biological corneal substitutes) have a less than 75% graft survival rate at 2 years. We aimed at developing a model for keratoprosthesis based on the use of poly(ethyl acrylate) (PEA)-based copolymers, extracellular matrix-protein coating and colonization with adipose-derived mesenchymal stem cells. Human adipose tissue derived mesenchymal stem cells (h-ADASC) colonization efficiency of seven PEA-based copolymers in combination with four extracellular matrix coatings were evaluated in vitro. Then, macroporous membranes composed of the optimal PEA subtypes and coating proteins were implanted inside rabbit cornea. After a 3-month follow-up, the animals were euthanized, and the clinical and histological biointegration of the implanted material were assessed. h-ADASC adhered and survived when cultured in all PEA-based macroporous membranes. The addition of high hydrophilicity to PEA membranes decreased h-ADASC colonization in vitro. PEA-based copolymer containing 10% hydroxyethyl acrylate (PEA-HEA10) or 10% acrylic acid (PEA-AAc10) monomeric units showed the best cellular colonization rates. Collagen plus keratan sulfate-coated polymers demonstrated enhanced cellular colonization respect to fibronectin, collagen, or uncoated PEAs. In vivo implantation of membranes resulted in an extrusion rate of 72% for PEA, 50% for PEA-AAc10, but remarkably of 0% for PEA-HEA10. h-ADASC survival was demonstrated in all the membranes after 3 months follow-up. A slight reduction in the extrusion rate of h-ADASC colonized materials was observed. No significant differences between the groups with and without h-ADASC were detected respect to transparency or neovascularization. We propose PEA with low hydroxylation as a scaffold for the anchoring ring of future keratoprosthesis.en
dc.description.statusPeer reviewed
dc.format.extent13
dc.identifier.citationDel Barrio , J L A , Chiesa , M , Ferrer , G G , Garagorri , N , Briz , N , Fernandez-Delgado , J , Valls , M S T , Botella , C C , García-Tuñón , I , Bataille , L , Rodriguez , A , Arnalich-Montiel , F , Ribelles , J L G , Antolinos-Turpín , C M , Gómez-Tejedor , J A , Alió , J L & De Miguel , M P 2015 , ' Biointegration of corneal macroporous membranes based on poly(ethyl acrylate) copolymers in an experimental animal model ' , Journal of Biomedical Materials Research - Part A , vol. 103 , no. 3 , pp. 1106-1118 . https://doi.org/10.1002/jbm.a.35249
dc.identifier.doi10.1002/jbm.a.35249
dc.identifier.issn1549-3296
dc.identifier.otherresearchoutputwizard: 11556/227
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=84923018966&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofJournal of Biomedical Materials Research - Part A
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subject.keywordsAdipose derived stem cells
dc.subject.keywordsCornea
dc.subject.keywordsCorneal scaffold
dc.subject.keywordsMacroporous membranes
dc.subject.keywordsPoly(ethyl acrylate)
dc.subject.keywordsMESENCHYMAL STEM-CELLS
dc.subject.keywordsHUMAN ADIPOSE-TISSUE
dc.subject.keywordsKERATOPROSTHESIS
dc.subject.keywordsCOLLAGEN
dc.subject.keywordsFIBRONECTIN
dc.subject.keywordsSCAFFOLDS
dc.subject.keywordsIMPLANT
dc.subject.keywordspoly(ethyl acrylate)
dc.subject.keywordsadipose derived stem cells
dc.subject.keywordscornea
dc.subject.keywordsmacroporous membranes
dc.subject.keywordscorneal scaffold
dc.subject.keywordsMESENCHYMAL STEM-CELLS
dc.subject.keywordsHUMAN ADIPOSE-TISSUE
dc.subject.keywordsKERATOPROSTHESIS
dc.subject.keywordsCOLLAGEN
dc.subject.keywordsFIBRONECTIN
dc.subject.keywordsSCAFFOLDS
dc.subject.keywordsIMPLANT
dc.subject.keywordsCeramics and Composites
dc.subject.keywordsBiomaterials
dc.subject.keywordsBiomedical Engineering
dc.subject.keywordsMetals and Alloys
dc.subject.keywordsFunding Info
dc.subject.keywordsSpanish Ministry of Health _x000D_ Spanish Ministry of Science and Innovation _x000D_ Fundacio Marato de TV3, Spain
dc.subject.keywordsSpanish Ministry of Health _x000D_ Spanish Ministry of Science and Innovation _x000D_ Fundacio Marato de TV3, Spain
dc.titleBiointegration of corneal macroporous membranes based on poly(ethyl acrylate) copolymers in an experimental animal modelen
dc.typejournal article
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