CHANGES IN OSTEOGENESIS BY HUMAN DENTAL PULP STEM CELLS ON PORCINE DECELLULARISED ADIPOSE TISSUE SOLID FOAMS OF DIFFERENT POROSITY

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dc.contributor.authorLuzuriaga, Jon
dc.contributor.authorGarcía-Urkia, Nerea
dc.contributor.authorSalvador-Moya, Jone
dc.contributor.authorPardo-Rodríguez, Beatriz
dc.contributor.authorEtxebarria, Iker
dc.contributor.authorFernandez-San-Argimiro, Francisco Javier
dc.contributor.authorOlalde, Beatriz
dc.contributor.authorUnda, Fernando
dc.contributor.authorPineda, Jose Ramon
dc.contributor.authorMadarieta, Iratxe
dc.contributor.authorIbarretxe, Gaskon
dc.contributor.institutionBiomateriales
dc.date.issued2023-07-01
dc.descriptionPublisher Copyright: © 2023, AO Research Institute Davos. All rights reserved.
dc.description.abstractThe extracellular matrix of white adipose tissue (AT) is a very promising biomaterial for tissue engineering, due to its abundance, easy accessibility, malleability, and proven biological activity. Decellularised AT (DAT) can be processed by freeze-drying in acetic acid solutions, and changing the DAT concentration in the solution gives rise to three-dimensional (3D) scaffolds of different stiffness and porosity. In a previous report, we demonstrated that human Dental Pulp Stem Cells (hDPSCs) could differentiate to osteoblasts and generate mineralised bone on 3D solid foams of porcine Decellularised Adipose Tissue (pDAT) at a concentration of 0.5 % (w/v). In this research work, we assessed whether and how osteogenesis by hDPSCs would be regulated by testing pDAT solid foams formulated at three different concentrations: 0.25 % (w/v), 0.5 % (w/v), and 1 % (w/v), which showed different stiffness, porosity and water retention properties. As a control condition we tested solid foams formulated with 0.5 % bovine Collagen-I. Thus, we performed Alkaline Phosphatase and Alizarin Red staining, together with Transmission Electron Microscopy and the detection of osteoblastic differentiation markers Osterix and Osteocalcin at both protein and transcript level, to compare the osteogenesis mediated by hDPSCs grown on all these 3D scaffolds, in the presence or absence of osteoblastic induction media. Our results demonstrate that pDAT at 0.25 % supported osteogenesis better than the rest of tested scaffolds, including bovine Collagen-I, in 3D hDPSC cultures. This enhanced osteogenesis could be attributed to the formulation of 0.25 % pDAT solid foams, which presented a higher porosity.en
dc.description.sponsorshipAuthors appreciate the kind technical assistance of Ricardo Andrade, Irene Fernández, and Alejandro Díez-Torre, staff members of the SGIKER service of Analytical Microscopy of UPV/EHU. Porosity analyses were performed by the Porous Solids Laboratory of the Central Research Support Services (SCAI) of the University of Malaga, Spain. This research was funded by the by the Basque Government (ELKARTEK program PLAKA KK-2019-00093, and Consolidated Research Groups IT1751-22) and the MCIN/AEI/10.13039/501100011033 -Spanish Ministry of Science and Innovation (PID2019-104766RB-C21).
dc.description.statusPeer reviewed
dc.format.extent19
dc.identifier.citationLuzuriaga , J , García-Urkia , N , Salvador-Moya , J , Pardo-Rodríguez , B , Etxebarria , I , Fernandez-San-Argimiro , F J , Olalde , B , Unda , F , Pineda , J R , Madarieta , I & Ibarretxe , G 2023 , ' CHANGES IN OSTEOGENESIS BY HUMAN DENTAL PULP STEM CELLS ON PORCINE DECELLULARISED ADIPOSE TISSUE SOLID FOAMS OF DIFFERENT POROSITY ' , European Cells and Materials , vol. 46 , pp. 119-137 . https://doi.org/10.22203/eCM.v046a06
dc.identifier.doi10.22203/eCM.v046a06
dc.identifier.issn1473-2262
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85180685828&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofEuropean Cells and Materials
dc.relation.projectIDEusko Jaurlaritza, IT1751-22-MCIN/AEI/10.13039/501100011033-PLAKA KK-2019-00093
dc.relation.projectIDEuskal Herriko Unibertsitatea, EHU
dc.relation.projectIDUniversiti Malaya, UM
dc.relation.projectIDMinisterio de Ciencia e Innovación, MICINN, PID2019-104766RB-C21
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subject.keywords3D culture
dc.subject.keywordsadipose tissue
dc.subject.keywordscell differentiation
dc.subject.keywordsdecellularisation
dc.subject.keywordsDental pulp stem cells
dc.subject.keywordsextracellular matrix
dc.subject.keywordsmineralisation
dc.subject.keywordsosteogenesis
dc.subject.keywordssolid foam
dc.subject.keywordsBioengineering
dc.subject.keywordsBiochemistry
dc.subject.keywordsBiomaterials
dc.subject.keywordsBiomedical Engineering
dc.subject.keywordsCell Biology
dc.titleCHANGES IN OSTEOGENESIS BY HUMAN DENTAL PULP STEM CELLS ON PORCINE DECELLULARISED ADIPOSE TISSUE SOLID FOAMS OF DIFFERENT POROSITYen
dc.typejournal article
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