Protein hydrolysate from organic fraction of municipal solid waste compost as nitrogen source to produce lactic acid by Lactobacillus fermentum ATCC 9338 and Lactobacillus plantarum NCIMB 8826

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dc.contributor.authorIzaguirre, Jon Kepa
dc.contributor.authorDietrich, Thomas
dc.contributor.authorVillarán, M. Carmen
dc.contributor.authorCastañón, Sonia
dc.contributor.institutionAlimentación Sostenible
dc.date.accessioned2024-07-24T12:02:53Z
dc.date.available2024-07-24T12:02:53Z
dc.date.issued2020-01
dc.descriptionPublisher Copyright: © 2019
dc.description.abstractIn this work a strategy for obtaining free amino-acids concentrate from an organic fraction of municipal solid waste compost and its use as a nitrogen source for lactic acid production, a compound widely used in different industries, using L. fermentum ATCC 9338 and L. plantarum NCIMB 8826 strains is described. Enzymatic digestion is based on the combined action of endoprotease Alcalase 1.5 MG and exoprotease Flavourzyme 500 MG. The highest degree of hydrolysis obtained under the optimal conditions was 41%. The use of glucanase Viscozyme L prior to protein hydrolysis helped to reduce the viscosity of the solution and promote the action of proteases, increasing its hydrolysis degree by 76%. The hydrolysate contained all 21 amino-acids, making it ideal for lactic acid bacteria growth. During shake flask cultivations the culture media was complemented with glucose as carbon source. Finally, with the hydrolysate, a maximum lactic acid concentration of 9.0 ± 0.2 g·L−1 and 11.1 ± 0.1 g·L−1 for L. fermentum ATCC 9338 and L. plantarum NCIMB 8826 respectively was obtained after 27 h. The innovation of the approach lies in exploiting the overproduction of compost for the production of lactic acid.en
dc.description.sponsorshipJon Kepa Izaguirre would like to acknowledge the Basque Government for awarding a predoctoral fellowship to achieve his PhD degree in the context of this research project. The authors are grateful to Dr. L. M. Quiros from Oviedo University for donating the strains L . fermentum ATCC 9338 and L . plantarum NCIMB 8826.
dc.description.statusPeer reviewed
dc.format.extent7
dc.identifier.citationIzaguirre , J K , Dietrich , T , Villarán , M C & Castañón , S 2020 , ' Protein hydrolysate from organic fraction of municipal solid waste compost as nitrogen source to produce lactic acid by Lactobacillus fermentum ATCC 9338 and Lactobacillus plantarum NCIMB 8826 ' , Process Biochemistry , vol. 88 , pp. 15-21 . https://doi.org/10.1016/j.procbio.2019.09.028
dc.identifier.doi10.1016/j.procbio.2019.09.028
dc.identifier.issn1359-5113
dc.identifier.urihttps://hdl.handle.net/11556/3292
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85072782245&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofProcess Biochemistry
dc.relation.projectIDEusko Jaurlaritza
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subject.keywordsCompost
dc.subject.keywordsEnzymatic hydrolysis
dc.subject.keywordsLactic acid
dc.subject.keywordsOrganic fraction municipal solid waste
dc.subject.keywordsProtein hydrolysate
dc.subject.keywordsBioengineering
dc.subject.keywordsBiochemistry
dc.subject.keywordsApplied Microbiology and Biotechnology
dc.subject.keywordsSDG 11 - Sustainable Cities and Communities
dc.subject.keywordsSDG 12 - Responsible Consumption and Production
dc.subject.keywordsSDG 9 - Industry, Innovation, and Infrastructure
dc.titleProtein hydrolysate from organic fraction of municipal solid waste compost as nitrogen source to produce lactic acid by Lactobacillus fermentum ATCC 9338 and Lactobacillus plantarum NCIMB 8826en
dc.typejournal article
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