Upgrading the organic fraction of municipal solid waste to poly(3-hydroxybutyrate)

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dc.contributor.authorIzaguirre, Jon Kepa
dc.contributor.authorda Fonseca, M. Manuela R.
dc.contributor.authorFernandes, Pedro
dc.contributor.authorVillarán, M. Carmen
dc.contributor.authorCastañón, Sonia
dc.contributor.authorCesário, M. Teresa
dc.contributor.institutionAlimentación Sostenible
dc.date.accessioned2024-07-24T12:00:11Z
dc.date.available2024-07-24T12:00:11Z
dc.date.issued2019-10
dc.descriptionPublisher Copyright: © 2019 Elsevier Ltd
dc.description.abstractThe organic fraction of municipal solid waste was studied as feedstock for the production of poly(3-hydroxybutyrate) (P(3HB)). To release the monosaccharides, a diluted acid pre-treatment followed by an enzymatic hydrolysis was applied. A sugar yield of 49% was achieved using a pre-treated waste and an enzyme cocktail of Pentopan 500 BG and Celluclast BG. The addition of Glucoamylase NS 22035 helped to hydrolyze the starch fraction, improving the hydrolysis yield to 56%. The hydrolysate was used as culture medium to produce P(3HB) by Burkholderia sacchari DSM 17165. Assays at shaking flask scale showed that when the hydrolysate was used as substrate, the attained cell concentration was slightly higher than in the control medium. It was necessary to supplement the hydrolysate with extra glucose to increase the C/N ratio and with a mineral solution to overcome the nutritional deficiencies. The P(3HB) accumulation using the supplemented hydrolysate was 58% (g polymer/g biomass).en
dc.description.sponsorshipThe authors are thankful for the technical and human support provided by SGIker of UPV/EHU and for the European funding ( ERDF and ESF ). Funding received by iBB-Institute for Bioengineering and Biosciences from FCT -Portuguese Foundation for Science and Technology (grant UID/BIO/04565/2013 ) and from Programa Operacional Regional de Lisboa 2020 (Project N. 007317) is acknowledged. Jon Kepa Izaguirre would like to acknowledge the Basque Government for awarding a predoctoral fellowship to achieve his PhD degree in the context of this research project. The authors are thankful for the technical and human support provided by SGIker of UPV/EHU and for the European funding (ERDF and ESF). Funding received by iBB-Institute for Bioengineering and Biosciences from FCT-Portuguese Foundation for Science and Technology (grant UID/BIO/04565/2013) and from Programa Operacional Regional de Lisboa 2020 (Project N. 007317) is acknowledged. Jon Kepa Izaguirre would like to acknowledge the Basque Government for awarding a predoctoral fellowship to achieve his PhD degree in the context of this research project.
dc.description.statusPeer reviewed
dc.identifier.citationIzaguirre , J K , da Fonseca , M M R , Fernandes , P , Villarán , M C , Castañón , S & Cesário , M T 2019 , ' Upgrading the organic fraction of municipal solid waste to poly(3-hydroxybutyrate) ' , Bioresource Technology , vol. 290 , 121785 . https://doi.org/10.1016/j.biortech.2019.121785
dc.identifier.doi10.1016/j.biortech.2019.121785
dc.identifier.issn0960-8524
dc.identifier.urihttps://hdl.handle.net/11556/3018
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85068834993&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofBioresource Technology
dc.relation.projectIDPrograma Operacional Regional de Lisboa 2020, 007317
dc.relation.projectIDFuel Cell Technologies Office, FCTO
dc.relation.projectIDFundação para a Ciência e a Tecnologia, FCT, UID/BIO/04565/2013
dc.relation.projectIDEusko Jaurlaritza
dc.relation.projectIDEuropean Regional Development Fund, FEDER, ESF
dc.relation.projectIDFundació Catalana de Trasplantament, FCT
dc.relation.projectIDInstitute for Bioengineering and Biosciences, iBB
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subject.keywordsBurkholderia sacchari
dc.subject.keywordsDiluted acid pre-treatment
dc.subject.keywordsEnzymatic hydrolysis
dc.subject.keywordsMunicipal solid waste
dc.subject.keywordsPoly(3-hydroxybutyrate)
dc.subject.keywordsBioengineering
dc.subject.keywordsEnvironmental Engineering
dc.subject.keywordsRenewable Energy, Sustainability and the Environment
dc.subject.keywordsWaste Management and Disposal
dc.subject.keywordsSDG 11 - Sustainable Cities and Communities
dc.subject.keywordsSDG 12 - Responsible Consumption and Production
dc.titleUpgrading the organic fraction of municipal solid waste to poly(3-hydroxybutyrate)en
dc.typejournal article
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