Genetic profiling of osteoblast-like cells cultured on a novel bone reconstructive material, consisting of poly-l-lactide, carbon nanotubes and microhydroxyapatite, in the presence of bone morphogenetic protein-2

Simple item page
dc.contributor.authorVan Der Zande, Meike
dc.contributor.authorWalboomers, X. Frank
dc.contributor.authorBrännvall, Mathias
dc.contributor.authorOlalde, Beatriz
dc.contributor.authorJurado, Maria J.
dc.contributor.authorÁlava, J. Iñaki
dc.contributor.authorJansen, John A.
dc.contributor.institutionBiomateriales
dc.contributor.institutionTecnalia Research & Innovation
dc.date.accessioned2024-07-24T12:15:26Z
dc.date.available2024-07-24T12:15:26Z
dc.date.issued2010-11
dc.description.abstractIn bone tissue engineering composite materials have been introduced, combining a degradable polymer matrix with, for instance, carbon nanotubes (CNTs) to improve mechanical properties or with microhydroxyapatite (μHA) to improve osteoconduction. The addition of bone morphogenetic protein-2 (BMP-2) can further improve the biological response to the material. However, the influence of such an elaborate composite formation on osteoprogenitor cells is unknown. To examine this, rat bone marrow (RBM) cells were cultured on porous poly-l-lactic acid and composite scaffolds, with or without added BMP-2. Cell proliferation and differentiation were studied using DNA, alkaline phosphatase and scanning electron microscopic analysis. Further, genetic profiles were examined by microarray investigation. Results showed that the composite scaffold had no significant effect on the proliferation of RBM cells, but indicated a negative effect on cell differentiation. The addition of BMP-2 also had no significant effect on the proliferation of RBM cells, but differentiation towards the osteogenic lineage was confirmed. In the arrays results, the addition of BMP-2 alone led to the expression of genes involved in (minor) inflammation. The composite scaffold, and even more distinctly the combination of the composite scaffold with BMP-2, led to the expression of genes, based on gene ontology, connected to tumorigenesis. Therefore, CNT- and μHA-containing composite materials are not recommended as a bone restorative material.en
dc.description.statusPeer reviewed
dc.format.extent9
dc.identifier.citationVan Der Zande , M , Walboomers , X F , Brännvall , M , Olalde , B , Jurado , M J , Álava , J I & Jansen , J A 2010 , ' Genetic profiling of osteoblast-like cells cultured on a novel bone reconstructive material, consisting of poly-l-lactide, carbon nanotubes and microhydroxyapatite, in the presence of bone morphogenetic protein-2 ' , Acta Biomaterialia , vol. 6 , no. 11 , pp. 4352-4360 . https://doi.org/10.1016/j.actbio.2010.06.013
dc.identifier.doi10.1016/j.actbio.2010.06.013
dc.identifier.issn1742-7061
dc.identifier.urihttps://hdl.handle.net/11556/4575
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=77956891981&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofActa Biomaterialia
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subject.keywordsBone morphogenetic protein-2 (BMP-2)
dc.subject.keywordsCarbon nanotubes
dc.subject.keywordsGenetic profiling
dc.subject.keywordsHydroxyapatite
dc.subject.keywordsIn vitro
dc.subject.keywordsBiotechnology
dc.subject.keywordsBiomaterials
dc.subject.keywordsBiochemistry
dc.subject.keywordsBiomedical Engineering
dc.subject.keywordsMolecular Biology
dc.titleGenetic profiling of osteoblast-like cells cultured on a novel bone reconstructive material, consisting of poly-l-lactide, carbon nanotubes and microhydroxyapatite, in the presence of bone morphogenetic protein-2en
dc.typejournal article
Files