Decellularization of xenografted tumors provides cell-specific in vitro 3D environment

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dc.contributor.authorIazzolino, Gaia
dc.contributor.authorMendibil, Unai
dc.contributor.authorArnaiz, Blanca
dc.contributor.authorRuiz-de-Angulo, Ane
dc.contributor.authorAzkargorta, Mikel
dc.contributor.authorUribe, Kepa B.
dc.contributor.authorKhatami, Neda
dc.contributor.authorElortza, Felix
dc.contributor.authorOlalde, Beatriz
dc.contributor.authorGomez-Vallejo, Vanessa
dc.contributor.authorLlop, Jordi
dc.contributor.authorAbarrategi, Ander
dc.contributor.institutionBiomateriales
dc.date.issued2022-08-18
dc.descriptionPublisher Copyright: Copyright © 2022 Iazzolino, Mendibil, Arnaiz, Ruiz-de-Angulo, Azkargorta, Uribe, Khatami, Elortza, Olalde, Gomez-Vallejo, Llop and Abarrategi.
dc.description.abstractIn vitro cell culture studies are common in the cancer research field, and reliable biomimetic 3D models are needed to ensure physiological relevance. In this manuscript, we hypothesized that decellularized xenograft tumors can serve as an optimal 3D substrate to generate a top-down approach for in vitro tumor modeling. Multiple tumor cell lines were xenografted and the formed solid tumors were recovered for their decellularization by several techniques and further characterization by histology and proteomics techniques. Selected decellularized tumor xenograft samples were seeded with the HCC1806 human triple-negative breast cancer (TNBC) basal-like subtype cell line, and cell behavior was compared among them and with other control 2D and 3D cell culture methods. A soft treatment using Freeze-EDTA-DNAse allows proper decellularization of xenografted tumor samples. Interestingly, proteomic data show that samples decellularized from TNBC basal-like subtype xenograft models had different extracellular matrix (ECM) compositions compared to the rest of the xenograft tumors tested. The in vitro recellularization of decellularized ECM (dECM) yields tumor-type–specific cell behavior in the TNBC context. Data show that dECM derived from xenograft tumors is a feasible substrate for reseeding purposes, thereby promoting tumor-type–specific cell behavior. These data serve as a proof-of-concept for further potential generation of patient-specific in vitro research models.en
dc.description.statusPeer reviewed
dc.format.extent1
dc.format.extent8764849
dc.identifier.citationIazzolino , G , Mendibil , U , Arnaiz , B , Ruiz-de-Angulo , A , Azkargorta , M , Uribe , K B , Khatami , N , Elortza , F , Olalde , B , Gomez-Vallejo , V , Llop , J & Abarrategi , A 2022 , ' Decellularization of xenografted tumors provides cell-specific in vitro 3D environment ' , Frontiers in Oncology , vol. 12 , 956940 , pp. 956940 . https://doi.org/10.3389/fonc.2022.956940
dc.identifier.doi10.3389/fonc.2022.956940
dc.identifier.issn2234-943X
dc.identifier.otherresearchoutputwizard: 11556/1409
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85137188627&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofFrontiers in Oncology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subject.keywordsTumor xenograft
dc.subject.keywordsDecellularization
dc.subject.keywordsIn vitro
dc.subject.keywords3D model
dc.subject.keywordsBreast cancer
dc.subject.keywordsTumor xenograft
dc.subject.keywordsDecellularization
dc.subject.keywordsIn vitro
dc.subject.keywords3D model
dc.subject.keywordsBreast cancer
dc.subject.keywordsOncology
dc.subject.keywordsCancer Research
dc.subject.keywordsSDG 3 - Good Health and Well-being
dc.subject.keywordsFunding Info
dc.subject.keywordsGrant RTI2018-101708-A-I00 funded by MCIN/AEI/10.13039/501100011033 and by ERDF A way of making Europe. Grants RYC2018-025502-I and PRE2018-084542 are funded by MCIN/AEI/10.13039/501100011033 and by ESF Investing in your future. Grant MDM-2017-0720 Maria de Maeztu Units of Excellence Program funded by the Spanish State Research Agency. Grant KK-2019/00093 Elkartek program funded by Basque Government. Grant CICBMG_PhD_03_2021 funded by CICbiomaGUNE and Polymat. Grant CICBMG_PhD_05_2019 funded by CICbiomaGUNE and Polymat. 2019 Leonardo Grant for Researchers and Cultural Creators, BBVA Foundation, grant number IN[19]_CMA_BIO_0119. The BBVA Foundation accepts no responsibility for the opinions, statements, and contents included, which are entirely the responsibility of the authors.
dc.subject.keywordsGrant RTI2018-101708-A-I00 funded by MCIN/AEI/10.13039/501100011033 and by ERDF A way of making Europe. Grants RYC2018-025502-I and PRE2018-084542 are funded by MCIN/AEI/10.13039/501100011033 and by ESF Investing in your future. Grant MDM-2017-0720 Maria de Maeztu Units of Excellence Program funded by the Spanish State Research Agency. Grant KK-2019/00093 Elkartek program funded by Basque Government. Grant CICBMG_PhD_03_2021 funded by CICbiomaGUNE and Polymat. Grant CICBMG_PhD_05_2019 funded by CICbiomaGUNE and Polymat. 2019 Leonardo Grant for Researchers and Cultural Creators, BBVA Foundation, grant number IN[19]_CMA_BIO_0119. The BBVA Foundation accepts no responsibility for the opinions, statements, and contents included, which are entirely the responsibility of the authors.
dc.titleDecellularization of xenografted tumors provides cell-specific in vitro 3D environmenten
dc.typejournal article
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