Enhanced Adipogenic Differentiation of Human Dental Pulp Stem Cells in Enzymatically Decellularized Adipose Tissue Solid Foams

Simple item page
dc.contributor.authorGarcia-Urkia, Nerea
dc.contributor.authorLuzuriaga, Jon
dc.contributor.authorUribe-Etxebarria, Veronica
dc.contributor.authorIrastorza, Igor
dc.contributor.authorFernandez-San-Argimiro, Francisco Javier
dc.contributor.authorOlalde, Beatriz
dc.contributor.authorBriz, Nerea
dc.contributor.authorUnda, Fernando
dc.contributor.authorIbarretxe, Gaskon
dc.contributor.authorMadarieta, Iratxe
dc.contributor.authorPineda, Jose Ramon
dc.contributor.institutionBiomateriales
dc.contributor.institutionSG
dc.date.issued2022-08
dc.descriptionPublisher Copyright: © 2022 by the authors.
dc.description.abstractEngineered 3D human adipose tissue models and the development of physiological human 3D in vitro models to test new therapeutic compounds and advance in the study of pathophysiological mechanisms of disease is still technically challenging and expensive. To reduce costs and develop new technologies to study human adipogenesis and stem cell differentiation in a controlled in vitro system, here we report the design, characterization, and validation of extracellular matrix (ECM)-based materials of decellularized human adipose tissue (hDAT) or bovine collagen-I (bCOL-I) for 3D adipogenic stem cell culture. We aimed at recapitulating the dynamics, composition, and structure of the native ECM to optimize the adipogenic differentiation of human mesenchymal stem cells. hDAT was obtained by a two-enzymatic step decellularization protocol and post-processed by freeze-drying to produce 3D solid foams. These solid foams were employed either as pure hDAT, or combined with bCOL-I in a 3:1 proportion, to recreate a microenvironment compatible with stem cell survival and differentiation. We sought to investigate the effect of the adipogenic inductive extracellular 3D-microenvironment on human multipotent dental pulp stem cells (hDPSCs). We found that solid foams supported hDPSC viability and proliferation. Incubation of hDPSCs with adipogenic medium in hDAT-based solid foams increased the expression of mature adipocyte LPL and c/EBP gene markers as determined by RT-qPCR, with respect to bCOL-I solid foams. Moreover, hDPSC capability to differentiate towards adipocytes was assessed by PPAR-γ immunostaining and Oil-red lipid droplet staining. We found out that both hDAT and mixed 3:1 hDAT-COL-I solid foams could support adipogenesis in 3D-hDPSC stem cell cultures significantly more efficiently than solid foams of bCOL-I, opening the possibility to obtain hDAT-based solid foams with customized properties. The combination of human-derived ECM biomaterials with synthetic proteins can, thus, be envisaged to reduce fabrication costs, thus facilitating the widespread use of autologous stem cells and biomaterials for personalized medicine.en
dc.description.sponsorshipThis research was funded by the Basque Government (IT1751-22; to G.I.; ELKARTEK program 566 PLAKA KK-2019-00093; to N.B.), the Health Department of the Basque Government (grant No. 2021333012; to J.R.P.), and grant No. RYC-2013-13450 and grant No. PID2019-104766RB-C21 funded by MCIN/AEI/10.13039/501100011033 by the European Union (NextGenerationEU) “Plan de Recuperación Transformación y Resiliencia” (grants to J.R.P.).
dc.description.statusPeer reviewed
dc.identifier.citationGarcia-Urkia , N , Luzuriaga , J , Uribe-Etxebarria , V , Irastorza , I , Fernandez-San-Argimiro , F J , Olalde , B , Briz , N , Unda , F , Ibarretxe , G , Madarieta , I & Pineda , J R 2022 , ' Enhanced Adipogenic Differentiation of Human Dental Pulp Stem Cells in Enzymatically Decellularized Adipose Tissue Solid Foams ' , Biology , vol. 11 , no. 8 , 1099 . https://doi.org/10.3390/biology11081099
dc.identifier.doi10.3390/biology11081099
dc.identifier.issn2079-7737
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85137362150&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofBiology
dc.relation.projectIDHealth Department of the Basque Government, PID2019-104766RB-C21-2021333012-RYC-2013-13450
dc.relation.projectIDEuropean Commission, EC
dc.relation.projectIDEusko Jaurlaritza, 566 PLAKA KK-2019-00093-IT1751-22
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subject.keywordsadipogenic differentiation
dc.subject.keywordsdecellularization
dc.subject.keywordsextracellular matrix
dc.subject.keywordshuman adipose tissue
dc.subject.keywordshuman dental pulp stem cells
dc.subject.keywordspersonalized medicine
dc.subject.keywordssolid foam
dc.subject.keywordsstem cell culture
dc.subject.keywordsGeneral Biochemistry,Genetics and Molecular Biology
dc.subject.keywordsGeneral Immunology and Microbiology
dc.subject.keywordsGeneral Agricultural and Biological Sciences
dc.titleEnhanced Adipogenic Differentiation of Human Dental Pulp Stem Cells in Enzymatically Decellularized Adipose Tissue Solid Foamsen
dc.typejournal article
Files