DNA copy number variation and gene expression analyses reveal the implication of specific oncogenes and genes in GBM

Margareto, Javier; Leis, Olatz; Larrarte, Eider; Pomposo, Iñigo C.; Garibi, Jesús María; Lafuente, Jose Vicenté

DNA copy number variation and gene expression analyses reveal the implication of specific oncogenes and genes in GBM

Identifiers

URI: https://hdl.handle.net/11556/3839

ISSN: 0735-7907

DOI: 10.1080/07357900802563044

Publication date

2009-06

Authors

Lafuente, Jose Vicenté

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Abstract

To understand the pathogenesis of glioblastoma multiforme (GBM) we used high-resolution comparative genomic hybridization arrays and gene expression microarrays to identify DNA copy number alterations and gene expression changes in comparable sets of GBM samples. Gains were detected at chromosomes 1, 2, 7, 9, 12, 19, and 20 and losses at 6, 9, and 10. Gene expression analyses identified specific genes overexpressed in GBM mapping at amplified chromosomal regions. Among these genes we found genes involved in angiogenesis, extracellular matrix remodeling and several oncogenes. DNA copy number analysis along with gene expression profiles provides a powerful strategy to understand tumor progression and identification of genes involved in GBM pathogenesis.

Keywords

CGH array , Copy number gains , Gene expression , Glioblastoma , Oncology , Cancer Research

Type

journal article

Citation

Margareto , J , Leis , O , Larrarte , E , Pomposo , I C , Garibi , J M & Lafuente , J V 2009 , ' DNA copy number variation and gene expression analyses reveal the implication of specific oncogenes and genes in GBM ' , Cancer Investigation , vol. 27 , no. 5 , pp. 541-548 . https://doi.org/10.1080/07357900802563044

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