Osteogenic differentiation of human dental pulp stem cells in decellularised adipose tissue solid foams

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dc.contributor.authorLuzuriaga, J
dc.contributor.authorGarcía-Gallastegui, P
dc.contributor.authorGarcía-Urkia, N
dc.contributor.authorPineda, JR
dc.contributor.authorIrastorza, I
dc.contributor.authorFernandez-San-Argimiro, F-J
dc.contributor.authorOlalde, B
dc.contributor.authorUnda, F
dc.contributor.authorMadarieta, I
dc.contributor.authorIbarretxe, G
dc.contributor.authorBriz, Nerea
dc.contributor.institutionBiomateriales
dc.contributor.institutionSG
dc.date.issued2022
dc.descriptionPublisher Copyright: © 2022, AO Research Institute Davos. All rights reserved.
dc.description.abstract3D cell culture systems based on biological scaffold materials obtainable from both animal and human tissues constitute very interesting tools for cell therapy and personalised medicine applications. The white adipose tissue (AT) extracellular matrix (ECM) is a very promising biomaterial for tissue engineering due to its easy accessibility, malleability and proven biological activity. In the present study, human dental pulp stem cells (hDPSCs) were combined in vitro with ECM scaffolds from porcine and human decellularised adipose tissues (pDAT, hDAT) processed as 3D solid foams, to investigate their effects on the osteogenic differentiation capacity and bone matrix production of hDPSCs, compared to single-protein-based 3D solid foams of collagen type I and conventional 2D tissue-culture-treated polystyrene plates. pDAT solid foams supported the osteogenic differentiation of hDPSCs to similar levels to collagen type I, as assessed by alkaline phosphatase and alizarin red stainings, reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) and osteocalcin/bone gamma-carboxyglutamate protein (BGLAP) immunostaining. Interestingly, hDAT solid foams showed a markedly lower capacity to sustain hDPSC osteogenic differentiation and matrix calcification and a higher capacity to support adipogenesis, as assessed by RT-qPCR and oil red O staining. White ATs from both human and porcine origins are relatively abundant and available sources of raw material to obtain high quality ECM-derived biomedical products. These biomaterials could have promising applications in tissue engineering and personalised clinical therapy for the healing and regeneration of lesions involving not only a loss of calcified bone but also its associated soft non-calcified tissues.en
dc.description.statusPeer reviewed
dc.format.extent18
dc.format.extent10730829
dc.identifier.citationLuzuriaga , J , García-Gallastegui , P , García-Urkia , N , Pineda , JR , Irastorza , I , Fernandez-San-Argimiro , F-J , Olalde , B , Unda , F , Madarieta , I , Ibarretxe , G & Briz , N 2022 , ' Osteogenic differentiation of human dental pulp stem cells in decellularised adipose tissue solid foams ' , European Cells and Materials , vol. 43 , pp. 112-129 . https://doi.org/10.22203/ecm.v043a10
dc.identifier.doi10.22203/ecm.v043a10
dc.identifier.issn1473-2262
dc.identifier.otherresearchoutputwizard: 11556/1400
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85126853056&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofEuropean Cells and Materials
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subject.keywordsDental pulp stem cells
dc.subject.keywordsAdipose tissue
dc.subject.keywordsBone tissue
dc.subject.keywordsExtracellular matrix
dc.subject.keywordsDecellularisation
dc.subject.keywordsSolid foam
dc.subject.keywordsOsteogenic differentiation
dc.subject.keywordsMineralisation
dc.subject.keywordsDental pulp stem cells
dc.subject.keywordsAdipose tissue
dc.subject.keywordsBone tissue
dc.subject.keywordsExtracellular matrix
dc.subject.keywordsDecellularisation
dc.subject.keywordsSolid foam
dc.subject.keywordsOsteogenic differentiation
dc.subject.keywordsMineralisation
dc.subject.keywordsBioengineering
dc.subject.keywordsBiochemistry
dc.subject.keywordsBiomaterials
dc.subject.keywordsBiomedical Engineering
dc.subject.keywordsCell Biology
dc.subject.keywordsFunding Info
dc.subject.keywordsThis research was supported by the Basque Government (ELKARTEK program PLAKA KK2019-00093; to NB), MICINN retos I+D+i (PID2019- 104766RB-C21, to JRP) and UPV/EHU (PPGA20/22; to FU, GI). The authors would like to thank the staff members of the SGIKER services of the UPV/EHU: Lipidomic service (Beatriz Abad) and Analytical Microscopy (Ricardo Andrade, Alejandro Díez-Torre and Irene Fernández) for their technical assistance.
dc.subject.keywordsThis research was supported by the Basque Government (ELKARTEK program PLAKA KK2019-00093; to NB), MICINN retos I+D+i (PID2019- 104766RB-C21, to JRP) and UPV/EHU (PPGA20/22; to FU, GI). The authors would like to thank the staff members of the SGIKER services of the UPV/EHU: Lipidomic service (Beatriz Abad) and Analytical Microscopy (Ricardo Andrade, Alejandro Díez-Torre and Irene Fernández) for their technical assistance.
dc.titleOsteogenic differentiation of human dental pulp stem cells in decellularised adipose tissue solid foamsen
dc.typejournal article
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