Browsing by Keyword "hydrolysis"
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Item Enzymic hydrolysis of animal fats in organic solvents at temperatures below their melting points(1991-05) Virto, M. D.; Lascaray, Jose Miguel; Solozabal, Rodolfo; de Renobales, Mertxe; Tecnalia Research & InnovationLipase from Candida rugosa catalyzed the hydrolysis of inedible beef tallow and pork lard (edible and inedible) in the presence of organic solvents at temperatures below the melting point of the fat. Reactions were carried out at 50% substrate with 180 lipase units per gram of fat in a two-liter reactor. In the presence of isooctane (5-10%) beef tallow yielded 94% hydrolysis in 24 hr both at 37° and 31°C. Edible pork lard yielded 97% hydrolysis under these conditions and at temperatures as low as 25°C, while inedible lard gave hydrolysis intermediate between the other two fats.Item Plasma polymerized silylated ciprofloxacin as an antibiotic coating(2011-07-22) Braceras, Inigo; Azpiroz, Patxi; Briz, Nerea; Fratila, Raluca M.; Oyarbide, Joseba; Ipiñazar, Enrique; Úlvarez, Noelia; Atorrasagasti, Garbiñe; Aizpurua, Jesus M.; INGENIERÍA DE SUPERFICIES; TECNOLOGÍAS DE HIDRÓGENO; SG; VALORIZACIÓN DE RESIDUOS; BiomaterialesLocally applied antibiotics under temporally controlled release present many advantages over systemic clinical treatments, e.g. efficiency and side effects. This can be achieved by a coating on top of the medical device, in which the antibiotic is stored. This study presents the use of plasma polymerization to produce such a coating using N,O-bis-tert- butyldimethylsilylated ciprofloxacin (silylciprofloxacin) as a precursor. Once exposed to physiological media, the outer layers of the coating release the antibiotic by a hydrolysis reaction. Thus, the plasma process parameters can control the speed of liberation through the coating polymerization. Besides, this study shows that the release products present antibiotic activity against a number of bacteria: E. coli, P. aeruginosa, and S. aureus. Ciprofloxacin release dynamics can be controlled by coating plasma polymerization parameters, allowing local controlled delivery of active antibiotic in physiologic conditions, and thus higher efficiency and lower side effects.Item Preparation of Hydrolyzed Sugarcane Molasses as a Low-Cost Medium for the Mass Production of Probiotic Lactobacillus paracasei ssp. paracasei F19(2023-01) Acosta-Piantini, Elsa; Rodríguez-Díez, Elena; Chavarri, María; López-de-Armentia, Iratxe; Villaran, M. Carmen; Lombraña, José Ignacio; Alimentación SostenibleIn this study, sugarcane molasses (SCM) was pre-treated in a low-cost fermentation medium to produce probiotic biomass of Lactobacillus paracasei ssp. paracasei F19 (LPPF19) with the combination of dilution, centrifugation, and acid hydrolysis (5 molar sulfuric acid, 60 °C/2 h). Microtox analysis, inductively coupled mass spectrometry (ICP-MS), and high-performance liquid chromatography (HPLC) were used to measure the effects of SCM pretreatment on the fermentation process. The results showed that the hydrolysis of sucrose into glucose and fructose was 98%, which represented an increase of 44.4% in the initial glucose content (fermentation-limiting sugar), and harmful heavy metals, such as arsenic, cadmium, and lead, were reduced by 50.3, 60.0, and 64.3%, respectively. After pretreatment, with the supplementation of only yeast extract and salts (Na, K, Mg, and Mn), a biomass of 9.58 log CFU/mL was achieved, approximately ten times higher than that for the control medium used (MRS/DeMan, Rogosa, and Sharpe). The cost reduction achieved compared to this commercial medium was 68.7% in the laboratory and 78.9% on an industrial scale. This work demonstrated that SCM could be used in a cheaper and more effective alternative fermentation to produce LPPF19.