Browsing by Keyword "Biocompatibility"
Now showing 1 - 4 of 4
Results Per Page
Sort Options
Item Bacterial adhesion reduction on a biocompatible Si+ ion implanted austenitic stainless steel(2011-10-10) Gallardo-Moreno, A. M.; Multigner, M.; Calzado-Martín, A.; Méndez-Vilas, A.; Saldaña, L.; Galván, J. C.; Pacha-Olivenza, M. A.; Perera-Núñez, J.; González-Carrasco, J. L.; Braceras, I.; Vilaboa, N.; González-Martín, M. L.; INGENIERÍA DE SUPERFICIESThe colonization of an implant surface by bacteria is an extremely important medical problem, which often leads to the failure of medical devices. Modern surface modification techniques, such as ion implantation, can confer to the surfaces very different properties from those of the bulk underlying material. In this work, austenitic stainless steel 316 LVM has been superficially modified by Si+ ion implantation. The effect of surface modification on the biocompatibility and bacterial adhesion to 316 LVM stainless steel has been investigated. To this aim, human mesenchymal stem cells (hMSCs), as precursor of osteoblastic cells, and bacterial strains relevant in infections related to orthopedic implants, i.e., Staphylococcus aureus and Staphylococcus epidermidis, have been assayed. For the understanding of changes in the biological response associated to ion implantation, variations in the chemical surface composition, topography, surface Gibbs energy, isoelectric point and in vitro corrosion behavior have been evaluated. hMSCs adhesion, viability and differentiation to the osteoblastic lineage were unaffected by Si+ ion implantation. On the other hand, Si+ ion implantation diminished the number of attached bacteria in static conditions and led to smaller adhesion rates and retention strength. The ability of implanted surfaces to reduce the bacterial adhesion was higher for Staphylococcus epidermidis than for Staphylococcus aureus. This study proposes Si+ ion implantation as an effective way of reducing bacterial adhesion on 316 LVM stainless steel surfaces without compromising its good biocompatibility.Item Decrease of Staphylococcal adhesion on surgical stainless steel after Si ion implantation(2014-08-15) Braceras, Iñigo; Pacha-Olivenza, Miguel A.; Calzado-Martín, Alicia; Multigner, Marta; Vera, Carolina; Broncano, Luis Labajos; Gallardo-Moreno, Amparo M.; González-Carrasco, José Luis; Vilaboa, Nuria; González-Martín, M. Luisa; INGENIERÍA DE SUPERFICIES; Biomateriales316LVM austenitic stainless steel is often the material of choice on temporal musculoskeletal implants and surgical tools as it combines good mechanical properties and acceptable corrosion resistance to the physiologic media, being additionally relatively inexpensive. This study has aimed at improving the resistance to bacterial colonization of this surgical stainless steel, without compromising its biocompatibility and resistance. To achieve this aim, the effect of Si ion implantation on 316LVM has been studied. First, the effect of the ion implantation parameters (50 keV; fluence: 2.5-5 × 10 16 ions/cm 2 ; angle of incidence: 45-90°) has been assessed in terms of depth profiling of chemical composition by XPS and nano-topography evaluation by AFM. The in vitro biocompatibility of the alloy has been evaluated with human mesenchymal stem cells. Finally, bacterial adhesion of Staphylococcus epidermidis and Staphylococcus aureus on these surfaces has been assessed. Reduction of bacterial adhesion on Si implanted 316LVM is dependent on the implantation conditions as well as the features of the bacterial strains, offering a promising implantable biomaterial in terms of biocompatibility, mechanical properties and resistance to bacterial colonization. The effects of surface composition and nano-topography on bacterial adhesion, directly related to ion implantation conditions, are also discussed.Item Fabio project: Development of innovative customized medical devices through new biomaterials and additive manufacturing technologies(Danube Adria Association for Automation and Manufacturing, DAAAM, 2010) Delgado, Javier; Blasco, Jose Ramon; Portoles, Luis; Ferris, Javier; Hurtos, Esther; Atorrasagasti, Garbine; BiomaterialesA goal in clinical practice is the total adaptation and personalization of medical devices to the special needs of the patient. Customization will be the best way to satisfy higher levels of quality, functionality, safety and biocompatibility during surgical treatment. Additive manufacturing technologies become the best alternative to produce customized medical devices. The FABIO project was proposed to develop new biomaterials and to adapt additive manufacturing technologies to obtain a new generation of customized medical devices. Four customized prototypes were designed, additively manufactured and validated within the project. Results have enabled the marketing of customized medical devices with high added value, great competitiveness and good levels of quality, functionality, safety and biocompatibility, in order to increase the satisfaction of the patient.Item Role of PLLA plasma surface modification in the interaction with human marrow stromal cells(2009-12-15) Armentano, Ilaria; Ciapetti, Gabriela; Pennacchi, Manuela; Dottori, Mariaserena; Devescovi, Valentina; Granchi, Donatella; Baldini, Nicola; Olalde, Beatriz; Jurado, Maria Jesus; Alava, Jose Inaki Marquinez; Kenny, José M.; Biomateriales; Tecnalia Research & InnovationThe effects of oxygen-based radio frequency plasma enhanced chemical vapor deposition (rf PECVD) on the surface of poly(L-lactide) (PLLA) polymers and the influence thereof on protein adsorption and on bone-cell behavior have been studied. Thin films and porous scaffolds based on PLLA polymer were developed, and the role of surface modifications were investigated extensively. PECVD surface treatments were used to alter surface functionality and modulate protein adsorption on the PLLA polymer matrix. In particular, Bovine Serum Albumine fluorescein isothiocyanate (fitc-BSA) conjugate adsorption on patterned surfaces of treated PLLA was analyzed by fluorescence microscopy. Human marrow stromal cells (MSCs) were cultured on scaffolds and cell adhesion and morphology were assessed using fluorescence microscopy. The results indicated that the PLLA surface became hydrophilic and its roughness increased with the treatment time and it had a dominant influence on the adsorption process of the protein. The outcome of the plasma treatment of various PLLA surfaces has been shown to be the up-regulator of the cell-adhesive proteins expression and consequently the improvement of cell adhesion and growth. Oxygen-treated PLLA promoted higher adhesion and proliferation of the MSCs in comparison to the untreated samples. It can be concluded that following plasma treatment, PLLA samples show enhanced affinity for osteoprogenitor cells.